Miki (Mitotic Kinetics Regulator) Immunoexpression in Normal Liver, Cirrhotic Areas and Hepatocellular Carcinomas: a Preliminary Study with Clinical Relevance
Hepatocellular carcinoma (HCC) is the most common type of primary malignant tumor in the liver. One of the main features of cancer survival is the generalized loss of growth control exhibited by cancer cells, and Miki is a protein related to the immunoglobulin superfamily that plays an important role in mitosis. We aim to study protein expression levels of Miki in non-tumoral liver and 20 HCCs recruited from a Pathology Department. Clinical information was also obtained. A tissue microarray was performed, and immunohistochemical techniques applied to study protein expression levels of Miki. In normal liver, Miki was weakly expressed, showing nuclear staining in the hepatocytes. Cirrhotic areas and HCCs showed a variety of staining patterns. Most HCC samples showed positive expression, with three different staining patterns being discernible: nuclear, cytoplasmic and mixed. Statistical analysis showed a significant association between grade of differentiation, Ki-67 proliferative index, survival rates and staining patterns. This study has revealed the positive expression of Miki in normal liver, cirrhotic areas and HCCs. Three different staining patterns of Miki expression with clinical relevance were noted in HCCs.
KeywordsHepatocellular carcinoma Miki Immunohistochemistry Liver Immunoglobulin superfamily
This work was supported by Pathology Department at Hospital Universitario de Araba-Txagorritxu.
IFV made the diagnoses of hepatocellular carcinomas, carried out the TMA preparations together with ECU, and coordinated the study. Immunohistochemistry was carried out by FBGC. Data analyses were performed by JSJ, and JJA. LLG, ECU and IFV carried out and interpreted the staining, contributed to data analyses and drafted the manuscript. BG, IGM and LMQ provided technical support and critically reviewed the manuscript. All authors read and approved the final manuscript.
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflicts of interest in the research.
- 11.Sugihara S, Nakashima O, Kojiro M, Majima Y, Tanaka M, Tanikawa K (1992) The morphologic transition in hepatocellular carcinoma. A comparison of the individual histologic features disclosed by ultrasound-guided fine-needle biopsy with those of autopsy. Cancer 70:1488–1492CrossRefPubMedGoogle Scholar
- 13.Matsuda Y, Yoshimura H, Ishiwata T, Sumiyoshi H, Matsushita A, Nakamura Y, et al (2016) Mitotic index and multipolar mitosis in routine histologic sections as prognostic markers of pancreatic cancers: a clinicopathological study. Pancreatology : official journal of the international association of Pancreatology16:127–132Google Scholar