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Integrated Bioinformatics Analysis of Potential Biomarkers for Prostate Cancer

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Pathology & Oncology Research

Abstract

The aim was to expound the pathogenesis of prostate cancer and to identify the potentially biomarkers for prostate cancer (PC). DNA methylation microarray data GSE38240 containing 8 prostate cancer metastases and 4 normal prostate samples as well as gene expression profile data GSE26910 containing 6 prostate primary tumors and 6 normal samples were used. Differentially expressed genes (DEGs) and differently methylated sites of PC were screened and the regulatory network was constructed with DEGs-related transcription factors (TFs). The obtained hub genes were subjected to protein-protein interaction network analysis. Enrichment analysis of down-regulated DEGs were performed. Total 351 DEGs including 190 down-regulated and 161 up-regulated genes and 3234 differently methylated sites were identified. In total 69 DEGs-related TFs were found. Regulatory network contained 1301 nodes and 2527 connection pairs and that FOXA1 (forkhead box A1), BZRAP1-AS1 (benzodiazapine receptor associated protein 1 antisense RNA 1) and KRT8 (keratin 8) were the top three nodes of it. The enriched GO terms were mainly biological activity of the blood and cells-related. Total 29 DEGs (such as AGTR1, angiotensin II receptor, type 1) and 57 none-DEGs involved in the PPI network. Biological functions in blood circulation and the involved AGTR1 may play important roles in PC by gene-methylation. Besides, BZRAP1-AS1 may be novel biomarker related with PC.

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Authors and Affiliations

  1. Department of urology, China-Japan Union Hospital of Jilin University, 126 Xiantai ST, Changchun, Jilin Province, 130033, China

    Jiufeng Tan, Xuefei Jin & Kaichen Wang

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  1. Jiufeng Tan
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  2. Xuefei Jin
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  3. Kaichen Wang
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Correspondence to Kaichen Wang.

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Tan, J., Jin, X. & Wang, K. Integrated Bioinformatics Analysis of Potential Biomarkers for Prostate Cancer. Pathol. Oncol. Res. 25, 455–460 (2019). https://doi.org/10.1007/s12253-017-0346-8

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  • DOI: https://doi.org/10.1007/s12253-017-0346-8

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