Abstract
MicroRNAs (miRNAs) are small non-coding RNAs composed of 18–25 nucleotides that can post-transcriptionally regulate gene expression and have key regulatory roles in cancer, acting as both oncogenes and tumor suppressors. About 1000 genes in humans encode miRNAs, which account for approximately 3% of the human genome, and up to 30% of human protein coding genes may be regulated by miRNAs. The objective of this article is to evaluate the expression profile of four miRNAs previously implicated in triple negative breast cancer: miR-10b, miR-26a, miR-146a and miR-153, and to determine their possible interaction in triple negative and non triple negative breast cancer based on clinical outcome and the expression of BRCA1. 24 triple-negative and 13 non triple negative breast cancer cases, were studied by q-RT-PCR and immunohistochemistry to determine the expression of the four studied miRNAs and the BRCA1 protein, respectively. We observed that the BRCA1 protein was absent in 62.5% of the triple negative cases. Besides, the miR-146a and miR-26a were over expressed in triple negative breast cancer. These two miRNAs, miR-10b and miR-153 were significantly associated to lymph node metastases occurrence in triple negative breast carcinoma. All the analyzed microRNAs were not associated with the expression of BRCA1 in our conditions. Our work provides evidence that miR-146a, miR-26a, miR-10b and miR-153 could be defined as biomarkers in triple negative breast cancer to predict lymph node metastases (LNM).
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Acknowledgements
We would like to thank Fabrice Kwiatkowski for his help in analysis data by SEM software.
This research was funded by grants from the Laboratory of Biochemistry Research unit UR 12ES08 Cell Signaling and Disease of Faculty of Medicine of Monastir, the department of Pathology of Farhat Hached Hospital, the Molecular Oncology Laboratory of Center Jean Perrin in Clermont-Ferrand and the Fuda (Founding of the University of Auvergne).
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Ethics approval and consent was waived by the institutional review board of the Farhat-Hached Hospital of Sousse (Comité d’éthique et de Recherche du CHU Farhat-Hached).
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Fkih M’hamed, I., Privat, M., Trimeche, M. et al. miR-10b, miR-26a, miR-146a And miR-153 Expression in Triple Negative Vs Non Triple Negative Breast Cancer: Potential Biomarkers. Pathol. Oncol. Res. 23, 815–827 (2017). https://doi.org/10.1007/s12253-017-0188-4
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DOI: https://doi.org/10.1007/s12253-017-0188-4