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Pathology & Oncology Research

, Volume 23, Issue 2, pp 323–328 | Cite as

Oroxylin a could be a Promising Radiosensitizer for Esophageal Squamous Cell Carcinoma by Inducing G2/M Arrest and Activating Apoptosis

  • Cheng Tan
  • Xia Qian
  • Yangyang Ge
  • Baixia Yang
  • Feng Wang
  • Zhifeng Guan
  • Jing CaiEmail author
Original Article

Abstract

To evaluate the radiosensitization of Oroxylin A on esophageal carcinoma cell as well as the optimal scheduling of Oroxylin A and radiotherapy (RT). Cell proliferation was estimated by a CCK8 assay. Radiosensitization was evaluated by a clonogenic survival assay. The progressions of Cell apoptosis and Cell cycle were investigated by flow cytometry. Expressions of survivin and cell cycle regulators were evaluated by Western blot analysis. A dose-dependent cell survival reduction was found in response to radiation with or without Oroxylin A. The apoptosis rates were remarkably dose-dependent higher in combination groups than in either Oroxylin A or radiation alone group. Besides, Oroxylin A could obviously radiosensitize ESCC cells by arresting tumor cells in G2/M phase and regulating cyclin B1 and Cdc 2 protein expression. Oroxylin A could be a promising radiosensitizer for esophageal squamous cell carcinoma by inducing G2/M phase blocking and activating cell apoptosis.

Keywords

Oroxylin A Radiosensitization ESCC Apoptosis G2/M arrest 

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Copyright information

© Arányi Lajos Foundation 2016

Authors and Affiliations

  • Cheng Tan
    • 1
  • Xia Qian
    • 1
  • Yangyang Ge
    • 1
  • Baixia Yang
    • 1
  • Feng Wang
    • 1
  • Zhifeng Guan
    • 1
  • Jing Cai
    • 1
    Email author
  1. 1.Department of Radiation Oncology, Nantong Tumor HospitalAffiliated Tumor Hospital of Nantong UniversityNantongChina

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