Pathology & Oncology Research

, Volume 23, Issue 2, pp 323–328 | Cite as

Oroxylin a could be a Promising Radiosensitizer for Esophageal Squamous Cell Carcinoma by Inducing G2/M Arrest and Activating Apoptosis

  • Cheng Tan
  • Xia Qian
  • Yangyang Ge
  • Baixia Yang
  • Feng Wang
  • Zhifeng Guan
  • Jing CaiEmail author
Original Article


To evaluate the radiosensitization of Oroxylin A on esophageal carcinoma cell as well as the optimal scheduling of Oroxylin A and radiotherapy (RT). Cell proliferation was estimated by a CCK8 assay. Radiosensitization was evaluated by a clonogenic survival assay. The progressions of Cell apoptosis and Cell cycle were investigated by flow cytometry. Expressions of survivin and cell cycle regulators were evaluated by Western blot analysis. A dose-dependent cell survival reduction was found in response to radiation with or without Oroxylin A. The apoptosis rates were remarkably dose-dependent higher in combination groups than in either Oroxylin A or radiation alone group. Besides, Oroxylin A could obviously radiosensitize ESCC cells by arresting tumor cells in G2/M phase and regulating cyclin B1 and Cdc 2 protein expression. Oroxylin A could be a promising radiosensitizer for esophageal squamous cell carcinoma by inducing G2/M phase blocking and activating cell apoptosis.


Oroxylin A Radiosensitization ESCC Apoptosis G2/M arrest 


  1. 1.
    Zali H, Ahmadi G, Bakhshandeh R, Rezaei-Tavirani M (2011) Proteomic analysis of gene expression during human esophagus cancer. J Paramedical Sci 2:37–44Google Scholar
  2. 2.
    Jemal A, Bray F, Center MM, Ferlay J, Ward E, Forman D (2011) Global cancer statistics. CA Cancer J Clin 61:69–90CrossRefPubMedGoogle Scholar
  3. 3.
    Thompson SK, Ruszkiewicz AR, Jamieson GG, Esterman A, Watson DI, Wijnhoven BP, et al. (2008) Improving the accuracy of TNM staging in esophageal cancer: a pathological review of resected specimens. Ann Surg Oncol 15(12):3447–3458CrossRefPubMedGoogle Scholar
  4. 4.
    Zhu H, Wang Q, Hu C, Zhang W, Quan L, Liu M, et al. (2011) High expression of survivin predicts poor prognosis in esophageal squamous cell carcinoma following radiotherapy. Tumour Biol 32:1147–1153CrossRefPubMedGoogle Scholar
  5. 5.
    Li J, Li ZN, Bao QL, Ge LP, Li XQ, Chen P (2012) Evaluation of pleural fluid survivin and XIAP for the diagnosis of malignant pleural effusion. Tumour Biol 33:1803–1810CrossRefPubMedGoogle Scholar
  6. 6.
    Salvesen GS, Duckett CSIAP (2002) Proteins: blocking the road to death’s door. Nat Rev Mol Cell Biol 3:401–410CrossRefPubMedGoogle Scholar
  7. 7.
    Wei L, Zhou Y, Qiao C, Ni T, Li Z, You Q, et al. (2015) Oroxylin a inhibits glycolysis-dependent proliferation of human breast cancer via promoting SIRT3-mediated SOD2 transcription and HIF1alpha destabilization. Cell Death Dis 6:e1714CrossRefPubMedPubMedCentralGoogle Scholar
  8. 8.
    Dinda B, SilSarma I, Dinda M, Rudrapaul P (2015) Oroxylum indicum (L.) Kurz, an important Asian traditional medicine: from traditional uses to scientific data for its commercial exploitation. J Ethnopharmacol 161:255–278CrossRefPubMedGoogle Scholar
  9. 9.
    Minsky BD, Pajak TF, Ginsberg RJ, et al. (2002) INT 0123 (Radiation Therapy Oncology Group 94–05) phase III trial of combinedmodality therapy for esophageal cancer: high-dose versus standarddoseradiation therapy. J Clin Oncol 20:1167–1174CrossRefPubMedGoogle Scholar
  10. 10.
    Zou M, Hu C, You Q, Zhang A, Wang X, Guo Q, Oroxylin A (2015) Induces autophagy in human malignant glioma cells via the mTOR-STAT3-notch signaling pathway. Mol Carcinog 54(11):1363–1375CrossRefPubMedGoogle Scholar
  11. 11.
    Wei L, Dai Q, Zhou Y, Zou M, Li Z, Lu N, et al. (2013) Oroxylin a sensitizes non-small cell lung cancer cells to anoikis via glucose-deprivation-like mechanisms: c-Src and hexokinase II. Biochim Biophys Acta 1830(6):3835–3845CrossRefPubMedGoogle Scholar
  12. 12.
    Cho HJ, YJ O, Han SH, Chung HJ, Kim CH, Lee NS, et al. (2013) Cdk1 protein-mediated phosphorylation of receptor-associated protein 80 (RAP80) serine 677 modulates DNA damage-induced G2/M checkpoint and cell survival. J Biol Chem 288(6):3768–3776CrossRefPubMedGoogle Scholar
  13. 13.
    Hengartner MO (2000) The biochemistry of apoptosis. Nature 407(6805):770–776CrossRefPubMedGoogle Scholar
  14. 14.
    Pawlik TM, Keyomarsi K (2004) Role of cell cycle in mediating sensitivity to radiotherapy. Int J Radiat Oncol Biol Phys 59(4):928–942CrossRefPubMedGoogle Scholar
  15. 15.
    Saez-Ayala M, Cabezas-Herrera J, Rodriguez-Lopez JN (2015) Targeting the epigenetic machinery of cancer cells. Oncogene 34(2):135–143CrossRefPubMedGoogle Scholar
  16. 16.
    Herscher LL (1999) Taxanes as radiosensitizers for head and neck cancer. Curr Opin Oncol 11(3):183–186CrossRefPubMedGoogle Scholar
  17. 17.
    Zhai X, Yang Y, Wan J, Zhu R, Inhibition WY (2013) Of LDH-A by oxamate induces G2/M arrest, apoptosis and increases radiosensitivity in nasopharyngeal carcinoma cells. Oncol Rep 30(6):2983–2991PubMedGoogle Scholar

Copyright information

© Arányi Lajos Foundation 2016

Authors and Affiliations

  • Cheng Tan
    • 1
  • Xia Qian
    • 1
  • Yangyang Ge
    • 1
  • Baixia Yang
    • 1
  • Feng Wang
    • 1
  • Zhifeng Guan
    • 1
  • Jing Cai
    • 1
    Email author
  1. 1.Department of Radiation Oncology, Nantong Tumor HospitalAffiliated Tumor Hospital of Nantong UniversityNantongChina

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