The anticancer effects of human amniotic membrane (hAM) have been studied over the last decade. However, the action mechanisms responsible for these effects are not fully understood until now. Previously results reported by our team proved that hAM is able to induce cytotoxicity and cell death in hepatocellular carcinoma (HCC), a worldwide high incident and mortal cancer. Therefore, this experimental study aimed to investigate the cellular targets of hAM protein extracts (hAMPE) in HCC through in vitro studies. Our results showed that hAMPE is able to modify oxidative stress environment in all HCC cell lines, as well as its cell cycle. hAMPE differently targets deoxyribonucleic acid (DNA), P21, P53, β-catenin and multidrug resistance (MDR) proteins in HCC cell lines. In conclusion, hAMPE has several targets in HCC, being clear that the success of this treatment depends of a personalized therapy based on the biological and genetic characteristics of the tumor.
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Ana Catarina Mamede (SFRH/BD/73649/2010) and Maria João Carvalho (SFRH/SINTD/60068/2009), wishes to thank to Portuguese Foundation for Science and Technology for their PhD Grants. The authors would also like to thank Obstetrics Service of Coimbra Hospital and University Centre for the collection of human tissues used in this work. Support: Santander Totta; Infarmed (Health Research Fund 2015); FCT, Portugal (Strategic Project PEst-C/SAU/UI3282/2013 and UID/NEU/04539/2013), COMPETE-FEDER.
All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.
Conflicts of Interest
The authors declare that they have no conflicts of interest.
Informed consent was obtained from all individual participants included in the study.
A. C. Mamede and S. Guerra contributed equally to this work.
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Mamede, A.C., Guerra, S., Laranjo, M. et al. Oxidative Stress, DNA, Cell Cycle/Cell Cycle Associated Proteins and Multidrug Resistance Proteins: Targets of Human Amniotic Membrane in Hepatocellular Carcinoma. Pathol. Oncol. Res. 22, 689–697 (2016). https://doi.org/10.1007/s12253-016-0053-x
- Human amniotic membrane
- Hepatocellular carcinoma
- Protein extracts
- Oxidative stress
- Cell cycle