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Expression of LC3, LAMP2, KEAP1 and NRF2 in Salivary Adenoid Cystic Carcinoma

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Pathology & Oncology Research

Abstract

Salivary Adenoid Cystic Carcinoma (SACC) is a tumor characterized by inevitable local progression and terminal hematogenous metastasis. This study aimed to investigate the expression of LC3, LAMP2, KEAP1 and NRF2 in SACC. Human salivary gland tissue microarray which contains 74 SACC, 12 pleomorphic adenoma and 18 normal salivary gland specimens. High expression of LC3, LAMP2, KEAP1 and NRF2 were found in SACC patients, and LC3, LAMP2, KEAP1 and NRF2 expression were significantly higher in SACC than as compared with pleomorphic adenoma and (or) normal salivary gland. The expression of NRF2 was correlated with pathological type of human SACC (P < 0.05). Moreover, the high-expression of KEAP1 had significant correlations with LC3 (P < 0.001, R = 0.3195), and LAMP2 (P < 0.001, R = 0.3346) and NRF2 (P < 0.05, R = 0.2246) by using the Pearson correlation coefficient test. Our findings demonstrated that up-regulation of LC3, LAMP2, KEAP1 and NRF2 were associated with carcinogenesis and progression of SACC patients, suggesting that they may be useful molecular targets in salivary adenoid cystic carcinoma.

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Acknowledgments

This study was supported by the National Natural Science Foundation of China 81072203, 81272963, 81472528 (ZJS), 81272964, 81472529 (WFZ), 81402241 (CFH) and the Fundamental Research Funds for the Central Universities (2042014kf0095).

Conflict of Interest Statement

The authors have declared that no competing interests exist.

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Authors and Affiliations

  1. The State Key Laboratory Breeding Base of Basic Science of Stomatology & Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, 37 Luoyu Road, Wuhan, 430079, People’s Republic of China

    Cong-Fa Huang, Wei-Wei Deng, Lu Zhang, Wen-Feng Zhang & Zhi-Jun Sun

  2. Department of Oral Maxillofacial-Head Neck Oncology, School and Hospital of Stomatology, Wuhan University, Wuhan, China

    Wen-Feng Zhang & Zhi-Jun Sun

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  1. Cong-Fa Huang
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  2. Wei-Wei Deng
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  3. Lu Zhang
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  4. Wen-Feng Zhang
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  5. Zhi-Jun Sun
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Corresponding authors

Correspondence to Wen-Feng Zhang or Zhi-Jun Sun.

Additional information

Cong-Fa Huang and Wei-Wei Deng contributed equally to this work.

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Huang, CF., Deng, WW., Zhang, L. et al. Expression of LC3, LAMP2, KEAP1 and NRF2 in Salivary Adenoid Cystic Carcinoma. Pathol. Oncol. Res. 22, 109–114 (2016). https://doi.org/10.1007/s12253-015-9981-0

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  • DOI: https://doi.org/10.1007/s12253-015-9981-0

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