Abstract
As lymphogenic dissemination is very common in melanoma, regional lymph nodes (LN)s represent first immunological barriers to tumor invasion and play a complex role in antitumor immune defense. In this sense, their most prominent role is the presentation of tumor-derived antigens to naïve T cells and generation of cell-mediated adaptive immune response. Since tumor micro-environment affects immune cell function in this study we have evaluated the ability of T cells and NK cells in metastatic (involved) and non-metastatic regional LNs to produce interferon γ (IFNγ), a pleiotropic cytokine that regulates adaptive antitumor immune response. Our results show reduced IFNγ production in both T and NK lymphocyte subsets and decreased prevalence of T cells in metastatic regional LNs of melanoma patients. The decrease of IFNγ production in T cells was more pronounced with increased number of involved regional LNs indicating tumor-induced functional impairment of both T and NK cell lymphocyte subsets in involved regional LNs. Therefore, shown low IFNγ production in metastatic LNs may represent an obstacle in adaptive cell-mediated antitumor immune response and hence may enable tumor progression.
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Acknowledgments
This study was supported by the grants of the Ministry of Science and Technology of the Republic of Serbia: grant number 41031 and grant number 175056. The authors wish to thank Jasna Popovic Basić for excellent technical assistance and help during this research. Special thanks we owe to doctors form the Department of Surgery at the Institute of Oncology and Radiology of Serbia Dr Neven Jokić, Dr Stevan Jokić, Dr Janko Pralica, Dr Zoran Kozomara, Dr Marko Jevrić, Dr Milan Žegarac, Dr Petar Radlović, Dr Milovan Juškić and Dr Predrag Radovanović.
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Vuletić, A., Jovanić, I., Jurišić, V. et al. Decreased Interferon γ Production in CD3+ and CD3−CD56+ Lymphocyte Subsets in Metastatic Regional Lymph Nodes of Melanoma Patients. Pathol. Oncol. Res. 21, 1109–1114 (2015). https://doi.org/10.1007/s12253-015-9938-3
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DOI: https://doi.org/10.1007/s12253-015-9938-3