Evaluation of Cell-Free Urine microRNAs Expression for the Use in Diagnosis of Ovarian and Endometrial Cancers. A Pilot Study
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Among gynaecological cancers, epithelial ovarian cancers are the most deadly cancers while endometrial cancers are the most common diseases. Efforts to establish relevant novel diagnostic, screening and prognostic markers are aimed to help reduce the high level of mortality, chemoresistance and recurrence, particularly in ovarian cancer. MicroRNAs, the class of post-transcriptional regulators, have emerged as the promising diagnostic and prognostic markers associated with various diseased states recently. Urine has been shown as the source of microRNAs several years ago; however, there has been lack of information on urine microRNA expression in ovarian and endometrial cancers till now. In this pilot study, we examined the expression of candidate cell-free urine microRNAs in ovarian cancer and endometrial cancer patients using quantitative real-time PCR. We compared the expression between pre- and post-surgery ovarian cancer samples, and between patients with ovarian and endometrial cancers and healthy controls, within three types of experiments. These experiments evaluated three different isolation methods of urine RNA, representing two supernatant and one exosome fractions of extracellular microRNA. In ovarian cancer, we found miR-92a significantly up-regulated, and miR-106b significantly down-regulated in comparison with control samples. In endometrial cancer, only miR-106b was found down-regulated significantly compared to control samples. Using exosome RNA, no significant de-regulations in microRNAs expression could be found in either of the cancers investigated. We propose that more research should now focus on confirming the diagnostic potential of urine microRNAs in gynaecological cancers using more clinical samples and large-scale expression profiling methods.
KeywordsOvarian cancer Endometrial cancer Urine microRNA miR-92a miR-106b
We are very grateful to Petra Soukupová, M.D., Laura Sucharovová, M.D. and Veronika Hanzíková, M.D. (Transfusion Department, General University Hospital Prague) involved in control patients sampling. We also thank Marta Číhalová, M.D. participating in sampling in FN Brno. We thank Jitka Pavlíková (FN Brno), Stanislava Štursová and Martina Vinopalová (Institute of the Care of Mother and Child Prague) ensuring samples collection. We are very grateful to Markéta Tesařová, Ph.D. (Department of Pediatrics and Adolescent Medicine 1. LF UK and VFN) allowing to analyse samples on Agilent 2100 Bioanalyzer. Performing Cy0 analyses by Cy0 team (www.cy0method.org) is greatly acknowledged, namely we thank Renato Panebianco for his kind cooperation. The financial support from the Charles University Prague (project PRVOUK-P27/LF1/1) is also greatly appreciated. Clinical part in FN Brno was supported by Ministry of Health of the Czech Republic – project FNBr 65269705.
Conflict of interest
The authors declare that they have no conflict of interest.
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