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Mutation Scanning of D1705 and D1709 in the RNAse IIIb Domain of MicroRNA Processing Enzyme Dicer in Cutaneous Melanoma

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Pathology & Oncology Research

Abstract

Since the discovery of microRNAs (miRNAs) there have been performed several studies showing perturbations in the expression of miRNAs and the miRNA expression machinery in cutaneous melanoma. Dicer, a pivotal cytosolic enzyme of miRNA maturation has shown to be affected by both somatic and germline mutations in a variety of cancers. Recent studies have shown that recurrent somatic mutations of Dicer frequently affect the metal-ion-binding sites D1709 and D1705 of its RNase IIIb domain, therefore called hot spot mutations. The present study investigates metal-ion-binding sites D1709 and D1705 of the Dicer RNase IIIb domain in cutaneous melanomas and melanoma metastasis by Sanger sequencing. All investigated samples showed wildtype sequence and no single mutation was detected. The miRNA processing enzyme Dicer of melanoma and melanoma metastasis does not appear to be affected by mutation in the metal-ion-binding sites D1709 and D1705 of its RNase IIIb domain.

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Authors and Affiliations

  1. Dermatologic Surgery Unit, Department of Dermatology, Venereology and Allergology, Ruhr-University Bochum, St. Josef Hospital, Gudrunstr. 56, 44791, Bochum, Germany

    Michael Sand, Falk G. Bechara, Marina Skrygan, Thilo Gambichler, Eggert Stockfleth & Schapoor Hessam

  2. Department of Plastic Surgery, St. Josef Hospital, Catholic Clinics of the Ruhr Peninsula, Heidbergweg 22-24, 45257, Essen, Germany

    Michael Sand & Michael Bromba

  3. University of Michigan Kellogg Eye Center, 1000 Wall Street, Ann Arbor, MI, 48105, USA

    Daniel Sand

Authors
  1. Michael Sand
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  2. Falk G. Bechara
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  3. Marina Skrygan
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  4. Daniel Sand
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  5. Thilo Gambichler
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  6. Michael Bromba
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  7. Eggert Stockfleth
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  8. Schapoor Hessam
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Correspondence to Michael Sand.

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Financial Disclosure

All authors hereby disclose any commercial associations that may pose or create a conflict of interest with the information presented in this manuscript. The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper. Daniel Sand is supported by the Heed Ophthalmic Foundation.

Ethics

This study conforms to the applicable local requirements regarding ethical and investigational committee review, informed consent, and other statutes or regulations regarding the protection of the rights and welfare of human subjects participating in medical research (Ethical Review Board of the Ruhr-University Bochum, Germany, registration number: 3265-08).

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Sand, M., Bechara, F.G., Skrygan, M. et al. Mutation Scanning of D1705 and D1709 in the RNAse IIIb Domain of MicroRNA Processing Enzyme Dicer in Cutaneous Melanoma. Pathol. Oncol. Res. 22, 639–641 (2016). https://doi.org/10.1007/s12253-015-0034-5

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  • DOI: https://doi.org/10.1007/s12253-015-0034-5

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