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Immunohistochemical Analysis of E-Cadherin, p53 and Inhibin-α Expression in Hydatidiform Mole and Hydropic Abortion

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Pathology & Oncology Research

Abstract

The purpose of this study was to investigate the role of E-cadherin, p53, and inhibin-α immunostaining in the differential diagnosis of hydropic abortion (HA), partial hydatidiform mole (PHM), and complete hydatidiform mole (CHM). E-cadherin, p53, and inhibin-α protein expression patterns were investigated immunohistochemically using paraffin -embedded tissue sections from histologically diagnosed cases of HA (n = 23), PHM (n = 24), and CHM (n = 23). Expression patterns of these markers were scored semi-quantitatively according to the staining intensity, percentage of positive cells, and immunoreactivity score. Classification of cases was established on histologic criteria and supported by the molecular genotyping. Immunostaining allowed the identification of specific cell types with E-cadherin, p53, and inhibin-α expression in all cases. E-cadherin expression was detected on the cell surface of villous cytotrophoblasts. We observed a marked decline in the expression of E-cadherin from HAs to PHMs to CHMs. The p53-positive reaction was restricted to the nucleus of villous cytotrophoblasts. Significantly increased p53 expression was observed in CHMs, compared with HAs and PHMs. The expression of inhibin-α was localised in the cytoplasm of villous syncytiotrophoblasts, and the expression of this marker was significantly higher in PHMs and CHMs than HAs. In conclusion, immunohistochemical analysis of E-cadherin, p53, and inhibin-α expression could serve as a useful adjunct to conventional methods in the differential diagnosis of HA, PHM, and CHM.

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References

  1. Cheung AN (2003) Pathology of gestational trophoblastic disease. Baillieres Best Pract Res Clin Obstet Gynaecol 17:849–868

    Article  Google Scholar 

  2. Fukunaga M, Katabuchi H, Nagasaka T, Mikami Y, Minamiguchi S, Lage JM (2005) Interobserver and intraobserver variability in the diagnosis of hydatidiform mole. Am J Surg Pathol 29:942–947

    Article  PubMed  Google Scholar 

  3. Jun SY, Ro JY, Kim KR (2003) P57kip2 is useful in the classification and differential diagnosis of complete and partial hydatidiform moles. Histopathology 43:17–25

    Article  PubMed  Google Scholar 

  4. Rowlands TM, Symonds JM, Farookhi R, Blaschuk OW (2000) Cadherins: crucial regulators of structure and function in reproductive tissues. Rev Reprod 5:53–61

    Article  CAS  PubMed  Google Scholar 

  5. Yuan W, Chen Z, Wu S, Ge J, Chang S, Wang X, Chen J, Chen Z (2009) Expression of EphA2 and E-cadherin in gastric cancer: correlated with tumor progression and lymphogenous metastasis. Pathol Oncol Res 15:473–478

    Article  CAS  PubMed  Google Scholar 

  6. Soussi T, Lozano G (2005) p53 mutation heterogeneity in cancer. Biochem Biophys Res Commun 331:834–842

    Article  CAS  PubMed  Google Scholar 

  7. Lapolt PS, Hsueh AJW (1991) Moleculer basis of inhibin production and action. Mol Cell Neurosci 2:449–463

    Article  CAS  PubMed  Google Scholar 

  8. Matias-Guiu X, Lerma E, Prat J (1998) Current topics in pathology of gynecologic tumors: a selective review. Int J Surg Pathol 6:121–134

    Article  Google Scholar 

  9. Stenvers KL, Findlay JK (2010) Inhibins: from reproductive hormones to tumor suppressors. Trends Endocrinol Metab 21:174–180

    Article  CAS  PubMed  Google Scholar 

  10. Wells M (2007) The pathology of gestational trophoblastic disease: recent advances. Pathology 39:88–96

    Article  PubMed  Google Scholar 

  11. Murphy KM, Mc Connell TG, Hafez MJ, Vang R, Ronnett BM (2009) Molecular genotyping of hydatidiform moles: analytic validation of a multiplex short tandem repeat assay. J Mol Diagn 11:598–605

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Hussein MR (2009) Analysis of p53, BCL-2 and epidermal growth factor receptor protein expression in the partial and complete hydatidiform moles. Exp Mol Pathol 87:63–69

    Article  CAS  PubMed  Google Scholar 

  13. Soper JT (2006) Gestational trophoblastic disease. Obstet Gynecol 108:176–187

    Article  PubMed  Google Scholar 

  14. Kimber SJ (2000) Molecular interactions at the maternal-embryonic interface during the early phase of implantation. Semin Reprod Med 18:237–253

    Article  CAS  PubMed  Google Scholar 

  15. Floridon C, Nielsen O, Holund B, Sunde L, Westergaard JG, Thomsen SG, Teisner B (2000) Localization of E-cadherin in villous, extravillous and vascular trophoblasts during intrauterine, ectopic and molar pregnancy. Mol Hum Reprod 6:943–950

    Article  CAS  PubMed  Google Scholar 

  16. Brown LM, Lacey HA, Baker PN, Crocker IP (2005) E-cadherin in the assessment of aberrant placental cytotrophoblast turnover in pregnancies complicated by preeclampsia. Histochem Cell Biol 124:499–506

    Article  CAS  PubMed  Google Scholar 

  17. Batistatou A, Makrydimas G, Zagorianakou N, Zagorianakou P, Nakanishi Y, Agnantis NJ, Hirohashi S, Charalabopoulos K (2007) Expression of dysadherin and E-cadherin in trophoblastic tissue in normal and abnormal pregnancies. Placenta 28:590–592

    Article  CAS  PubMed  Google Scholar 

  18. Shu H, Chen H, Yang B, Chang Z, Xiong M, Chen W (2013) Aberrant expression of E-cadherin and integrin β-1 in trophoblasts is associated with malignant gestational trophoblastic diseases. Int J Gynecol Cancer 23:749–754

    Article  PubMed  Google Scholar 

  19. Xue WC, Feng HC, Tsao SW, Chan KY, Ngan HY, Chiu PM, Maccalman CD, Cheung AN (2003) Methylation status and expression of E-cadherin and cadherin-11 in gestational trophoblastic diseases. Int J Gynecol Cancer 13:879–888

    Article  CAS  PubMed  Google Scholar 

  20. Balaram P, Alex S, Panikkar B, Rajalekshmi TN (2004) Adhesion-related proteins E-cadherin, P-cadherin, CD44, and CD44v6, and antimetastatic protein nm23H1 in complete hydatidiform moles in relation to invasion potential. Int J Gynecol Cancer 14:532–539

    Article  CAS  PubMed  Google Scholar 

  21. Cohen M, Meisser A, Haenggeli L, Irminger-Finger I, Bischof P (2007) Status of p53 in first-trimester cytotrophoblastic cells. Mol Hum Reprod 13:111–116

    Article  CAS  PubMed  Google Scholar 

  22. Marzusch K, Ruck P, Horny HP, Dietl J, Kaiserling E (1995) Expression of the p53 tumour suppressor gene in human placenta: an immunohistochemical study. Placenta 16:101–104

    Article  CAS  PubMed  Google Scholar 

  23. Halperin R, Peller S, Sandbank J, Bukovsky I, Schneider D (2000) Expression of the p53 gene and apoptosis in gestational trophoblastic disease. Placenta 21:58–62

    Article  CAS  PubMed  Google Scholar 

  24. Kale A, Söylemez F, Ensari A (2001) Expressions of proliferation markers (Ki-67, proliferating cell nuclear antigen, and silver-staining nucleolar organizer regions) and of p53 tumor protein in gestational trophoblastic disease. Am J Obstet Gynecol 184:567–574

    Article  CAS  PubMed  Google Scholar 

  25. Petignat P, Laurini R, Goffin F, Bruchim I, Bischof P (2006) Expression of matrix metalloproteinase-2 and mutant p53 is increased in hydatidiform mole as compared with normal placenta. Int J Gynecol Cancer 16:1679–1684

    Article  CAS  PubMed  Google Scholar 

  26. Al-Bozom IA (2000) p53 and Bcl-2 oncoprotein expression in placentas with hydropic changes and partial and complete moles. APMIS 108:756–760

    Article  CAS  PubMed  Google Scholar 

  27. Chen Y, Shen D, Gu Y, Zhong P, Xie J, Song Q (2012) The diagnostic value of Ki-67, P53 and P63 in distinguishing partial hydatidiform mole from hydropic abortion. Wien Klin Wochenschr 124:184–187

    Article  CAS  PubMed  Google Scholar 

  28. Petraglia F (1997) Inhibin, activin, and follistatin in the human placenta: a new family of regulatory proteins. Placenta 18:3–8

    Article  CAS  PubMed  Google Scholar 

  29. Mylonas I, Jeschke U, Wiest I, Hoeing A, Vogl J, Shabani N, Kuhn C, Schulze S, Kupka MS, Friese K (2004) Inhibin/activinsubunits alpha, beta-A and beta-B are differentially expressed in normal human endometrium throughout the menstrual cycle. Histochem Cell Biol 122:461–471

    Article  CAS  PubMed  Google Scholar 

  30. de Kretser DM, HedgerMP LKL, Phillips DJ (2002) Inhibins, activins and follistatin in reproduction. Hum Reprod Update 8:529–541

    Article  PubMed  Google Scholar 

  31. Mylonas I, Schiessl B, Jeschke U, Vogl J, Makrigiannakis A, Kuhn C, Schulze S, Kainer F, Friese K (2006) Expression of inhibin/activin subunits alpha (−alpha), betaA (−betaA), and betaB (betaB) in placental tissue of normal, preeclamptic, and HELLP pregnancies. Endocr Pathol 17:19–33

    Article  CAS  PubMed  Google Scholar 

  32. Shih IM, Kurman RJ (1999) Immunohistochemical localization of inhibin-alpha in the placenta and gestational trophoblastic lesions. Int J Gynecol Pathol 18:144–150

    Article  CAS  PubMed  Google Scholar 

  33. Kommoss F, Schmidt D, Coerdt W, Olert J, Müntefering H (2001) Immunohistochemical expression analysis of inhibin-alpha and -beta subunits in partial and complete moles, trophoblastic tumors, and endometrial decidua. Int J Gynecol Pathol 20:380–385

    Article  CAS  PubMed  Google Scholar 

  34. Mylonas I, Shabani N, Vogl J, Makovitzky J, Kunze S, Kuhn C, Schulze S, Friese K, Jeschke U (2007) Inhibin/activin subunits are immunohistochemically expressed in complete and partial hydatidiform moles. Anticancer Res 27:1995–2000

    CAS  PubMed  Google Scholar 

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Acknowledgments

This study was supported by the Scientific Research Fund of Antalya Training and Research Hospital under project number 2013–117082.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Antalya Training and Research Hospital, Antalya, Turkey

    Onur Erol, Aysel Uysal Derbent & Mustafa Kemal Özel

  2. Department of Pathology, Antalya Training and Research Hospital, Antalya, Turkey

    Dinç Süren, Birsel Tutuş, Ahmet Arda Gökay & Cem Sezer

  3. Department of Gynecologic Oncology, Antalya Training and Research Hospital, Antalya, Turkey

    Tayfun Toptaş

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  1. Onur Erol
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  2. Dinç Süren
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  3. Birsel Tutuş
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  5. Ahmet Arda Gökay
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  6. Aysel Uysal Derbent
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Correspondence to Onur Erol.

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Erol, O., Süren, D., Tutuş, B. et al. Immunohistochemical Analysis of E-Cadherin, p53 and Inhibin-α Expression in Hydatidiform Mole and Hydropic Abortion. Pathol. Oncol. Res. 22, 515–521 (2016). https://doi.org/10.1007/s12253-015-0031-8

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  • DOI: https://doi.org/10.1007/s12253-015-0031-8

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