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The Level of ALR is Regulated by the Quantity of Mitochondrial DNA

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Pathology & Oncology Research

Abstract

Augmenter of liver regeneration (ALR) contributes to mitochondrial biogenesis, maintenance and to the physiological operation of mitochondria. The depletion of ALR has been widely studied and had serious consequences on the mitochondrial functions. However the inverse direction, the effect of the depletion of mitochondrial electron transfer chain and mtDNA on ALR expression has not been investigated yet. Thus mtDNA depleted, ρ0 cell line was prepared to investigate the role of mitochondrial electron transfer chain and mtDNA on ALR expression. The depletion of mtDNA has not caused any difference at mRNA level, but at protein level the expression of ALR has been markedly increased. The regulatory role of ATP and ROS levels could be ruled out because the treatment of the parental cell line with different respiratory inhibitors and uncoupling agent could not provoke any changes in the protein level of ALR. The effect of mtDNA depletion on the protein level of ALR has been proved not to be liver specific, since the phenomenon could be observed in the case of two other, non-hepatic cell lines. It seems the level of mtDNA and/or its products may have regulatory role on the protein level of ALR. The up-regulation of ALR can be a part of the adaptive response in ρ0 cells that preserves the structural integrity and the transmembrane potential despite the absence of protein components encoded by the mtDNA.

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Acknowledgments

This work was financially supported by National Scientific Research Fund grant (OTKA 105416) and MedinProt Protein Excellence foundation. Tamás Lőrincz is a Gedeon Richter Plc Talentum fellowship recipient.

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Authors and Affiliations

  1. Department of Applied Biotechnology and Food Science, Laboratory of Biochemistry and Molecular Biology, Budapest University of Technology and Economics, Szent Gellért tér 4, Budapest, 1111, Hungary

    Tibor Balogh, Tamás Lőrincz & András Szarka

  2. Pathobiochemistry Research Group of Hungarian Academy of Sciences, Semmelweis University, P.O. Box 260, Budapest, 1444, Hungary

    Ibolya Stiller & András Szarka

  3. Department of Medical Chemistry, Molecular Biology and Pathobiochemistry Pathobiochemistry, Semmelweis University, POB 260, Budapest, 1444, Hungary

    József Mandl & Gábor Bánhegyi

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  1. Tibor Balogh
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  2. Tamás Lőrincz
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  3. Ibolya Stiller
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  4. József Mandl
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  5. Gábor Bánhegyi
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  6. András Szarka
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Correspondence to András Szarka.

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Balogh, T., Lőrincz, T., Stiller, I. et al. The Level of ALR is Regulated by the Quantity of Mitochondrial DNA. Pathol. Oncol. Res. 22, 431–437 (2016). https://doi.org/10.1007/s12253-015-0020-y

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  • DOI: https://doi.org/10.1007/s12253-015-0020-y

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