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Polycomb Group Oncogene RING1 is Over-expressed in Non-Small Cell Lung Cancer

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Pathology & Oncology Research

Abstract

Ring finger protein 1 (RING1) have recently been reported to be related to aggressive tumor features in Prostate Cancer and urothelial carcinoma of the bladder. However, the role of RING1 in non-small-cell lung cancer (NSCLC) tumorigenesis has never been elucidated. So we aimed at investigating the potential role of RING1 in NSCLC. RING1 expression was evaluated by Immunoblot in 8 paired fresh lung cancer tissues and immunohistochemistry on 69 paraffin-embedded sections from 2006 to 2009. Furthermore, flow-cytometry and RNA interference were performed to analyse the role of RING1 in A549 cells. We showed that the expression level of RING1 was significant increased in lung cancer as compared with the adjacent normal tissue. High expression level of RING1 was associated with TNM stage (P = 0.013), and RING1 was positively related with proliferation marker Ki67 (P < 0.05). Moreover, RING1 knockdown induces growth suppression of human lung cancer cells through G1/S cell cycle phase arrest in vitro. Kaplan–Meier survival curves showed that high expression level of RING1 was associated with poor prognosis (P = 0.03). On the basis of these results, we suggested that RING1 protein expression may be a favorable independent prognostic parameter for non-small cell lung cancer.

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Abbreviations

NSCLC:

Non-small cell lung cancer

PcG:

Poly-comb group

CDK2:

Cyclin-dependent kinase 2

GWAS:

Genome-wide association studies

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Acknowledgments

This work was supported by the National Natural Scientific Foundation of China (no. 81272708).

Conflict of interest

None.

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Authors and Affiliations

  1. Department of Thoracic Surgery, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China

    Yiqun Zhou, Yangcheng Li, Dunpeng Yang & Qun Xue

  2. Department of Public Health, Nantong University, Nantong, 226001, Jiangsu, China

    Chunhua Wan

  3. Department of Oncology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China

    Yifei Liu, Liting Lv, Buyou Chen, Xiaodong Zheng & Guoxin Mao

  4. Department of Gastroenterology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China

    Runzhou Ni & Yuexia Huang

  5. Nantong University, 20 Xisi Road, Nantong, Jiangsu Province, 226001, China

    Guoxin Mao & Qun Xue

Authors
  1. Yiqun Zhou
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  2. Chunhua Wan
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  3. Yifei Liu
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  10. Dunpeng Yang
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  11. Guoxin Mao
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Corresponding authors

Correspondence to Guoxin Mao or Qun Xue.

Additional information

Yiqun Zhou and Chunhua Wan contributed equally to this work.

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Zhou, Y., Wan, C., Liu, Y. et al. Polycomb Group Oncogene RING1 is Over-expressed in Non-Small Cell Lung Cancer. Pathol. Oncol. Res. 20, 549–556 (2014). https://doi.org/10.1007/s12253-013-9727-9

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  • DOI: https://doi.org/10.1007/s12253-013-9727-9

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