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Prognostic Significance of Human Papillomavirus (HPV) Status and Expression of Selected Markers (HER2/neu, EGFR, VEGF, CD34, p63, p53 and Ki67/MIB-1) on Outcome After (Chemo-) Radiotherapy in Patients with Squamous Cell Carcinoma of Uterine Cervix

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Pathology & Oncology Research

Abstract

The aim of the retrospective study was to evaluate prognostic significance of human papillomavirus (HPV) status and expression of epidermal growth factor receptor (EGFR), human epidermal growth factor receptor type 2 (HER2/neu), vascular endothelial growth factor (VEGF), CD34 antigen, tumor suppressors p63 and p53, and Ki67/MIB-1 in squamous cell carcinoma of the uterine cervix (SCCC) treated with radiotherapy or chemoradiotherapy. Seventy-two consecutive patients with SCCC, diagnosed and treated with (chemo-) radiotherapy with a curative intent at the University Hospital Hradec Kralove between August 1998 and August 2008, were enrolled in the study. The median follow-up period was 57 months (range 5–152). The tested biological factors were evaluated by polymerase chain reaction (HPV status) and by immunohistochemistry (remaining above mentioned markers) from archival paraffin embedded original diagnostic tumor samples. A statistical significant correlation was observed between low expression of p63 and poor overall survival (p = 0.001), although the complete response probability was influenced with borderline statistical significance (p = 0.05). However, the results could be affected by the statistical error due to the small number of p63 negative patients. HPV positivity and EGFR staining intensity was associated with higher complete response probability (p = 0.038 and p = 0.044, resp.). All other results were not significant. Neither HPV positivity nor EGFR staining intensity were reflected in the overall survival evaluation. In conclusion, the presented study did not confirm any apparently significant association of the suggested markers with prognosis of SCCC in patients treated with (chemo-) radiotherapy.

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Acknowledgments

The study was supported by Internal Grant Agency of Ministry of Health of Czech Republic NT11334-4/2010, by the programme PRVOUK P37/06, and by project LM2010004.

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Authors and Affiliations

  1. Department of Oncology and Radiotherapy, University Hospital Hradec Kralove, Sokolská 581, 500 05, Hradec Kralove, Czech Republic

    Milan Vosmik

  2. University Hospital Hradec Kralove, Hradec Kralove, Czech Republic

    Jan Laco, Igor Sirak, Martin Beranek, Eva Hovorkova, Hana Vosmikova, Monika Drastikova, Miroslav Hodek, Zdenek Zoul, Karel Odrazka & Jiri Petera

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  1. Milan Vosmik
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Correspondence to Milan Vosmik.

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Vosmik, M., Laco, J., Sirak, I. et al. Prognostic Significance of Human Papillomavirus (HPV) Status and Expression of Selected Markers (HER2/neu, EGFR, VEGF, CD34, p63, p53 and Ki67/MIB-1) on Outcome After (Chemo-) Radiotherapy in Patients with Squamous Cell Carcinoma of Uterine Cervix. Pathol. Oncol. Res. 20, 131–137 (2014). https://doi.org/10.1007/s12253-013-9674-5

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  • DOI: https://doi.org/10.1007/s12253-013-9674-5

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