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Tumor-Related Gene Changes in Immunosuppressive Syrian Hamster Cholangiocarcinoma

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Pathology & Oncology Research

Abstract

The results of a previous study demonstrated that prednisolone enhanced cholangiocarcinogenesis. Therefore, to clarify molecular changes during immunosuppressive cholangiocarcinogenesis, Syrian hamsters were divided into 8 groups: uninfected controls; immunosuppressed Syrian hamsters using prednisolone (P); normal Syrian hamsters administered N-nitrosodimethylamine (ND); immunosuppressed Syrian hamsters administered N-nitrosodimethylamine (NDis); normal Syrian hamsters infected with Opisthorchis viverrini (OV); immunosuppressed Syrian hamsters infected with O. viverrini (OVis); normal Syrian hamsters infected with O. viverrini and administered N-nitrosodimethylamine (CCA); and immunosuppressed Syrian hamsters infected with O. viverrini and administered N-nitrosodimethylamine (CCAis). Syrian hamster livers were used for analysis of tumor-related gene expression and immunohistochemistry through cytokeratin 19 (CK19) and proliferating cell nuclear antigen (PCNA) staining. The tumor-related gene expression results show that CCAis groups at all time points exhibited upregulation of COX-2, IL-6, SOD1, CAT and iNOS and downregulation of p53, which correlated with the predominant expression of CK19 and PCNA in liver tissue. These results suggest that prednisolone enhances cholangiocarcinoma development, which was confirmed by molecular changes.

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Acknowledgments

This research was supported by grant from under the program Strategic Scholarships for Frontier Research Network for the Ph.D. Program Thai Doctoral degree from the Office of the Higher Education Commission, Thailand. The authors also gratefully acknowledge financial support from the Higher Education Research Promotion and National Research University Project of Thailand, Office of the Higher Education Commission, through the Health Cluster (SHep-GMS), Khon Kaen University. We also wish to thank the Department of Research affair (AS54301), Animal Experimental Unit, Faculty of Medicine, Khon Kaen University, for their assistance.

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Juasook, A., Aukkanimart, R., Boonmars, T. et al. Tumor-Related Gene Changes in Immunosuppressive Syrian Hamster Cholangiocarcinoma. Pathol. Oncol. Res. 19, 785–794 (2013). https://doi.org/10.1007/s12253-013-9645-x

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  • DOI: https://doi.org/10.1007/s12253-013-9645-x

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