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Prolyl Isomerase Pin1 Regulated Signaling Pathway Revealed by Pin1 +/+ and Pin1 −/− Mouse Embryonic Fibroblast Cells

Pathology & Oncology Research volume 19, pages 667–675 (2013)Cite this article

Abstract

Pin1 (peptidylprolyl cis/trans isomerase, NIMA-interacting 1) plays a key role in a number of diseases including cancer and Alzheimer disease. Previous studies have identified a wide range of phosphoproteins as Pin1 substrates. Related pathways were analyzed separately. The aim of this study was to provide a comprehensive picture involving Pin1 regulation. A genome-wide mRNA expression microarray was carried out using the RNA isolation from Pin1 +/+ and Pin1 −/− mouse embryonic fibroblast (MEF) cells. Signaling pathways regulated by Pin1 were analyzed with the utility of KEGG pathway and GO annotation. An expression pattern regulated by Pin1 was revealed. A total of 606 genes, 375 being up-regulated and 231 down-regulated, were differentially expressed when comparing Pin1 +/+ to Pin1 −/− MEF cells. Totally 48 pathways were shown to be regulated by Pin1 expression in KEGG pathway analysis. In the GO annotation system, 19 processes on biological processes, 15 processes on cellular components, and 18 processes on molecular functions were found to be in the regulation of Pin1 expression. Pathways related to immune system and cancer showed most significant association with Pin1 regulation. Pin1 is an important regulator in a wide range of signaling pathways that were related to immune system and cancer.

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Acknowledgments

This work was supported by National Natural Science Foundation of China (No: 30672379 and No: 30973374 to Z H), Natural Science Foundation of Guangdong Province (to Z H), Science and Technology Innovation Fund of Guangdong Medical College (No: STIF201108 to Z H), Medical Science Research Foundation of Guangdong Province (No: B2011232 to G-L H), and Science Research Foundation of Guangdong Medical College (No: B2010003 to G-L H).

Author Contributions

Experiment and data analysis: Guo-Liang Huang, Jin-Hua Qiu, Bin-Bin Li, Jing-Jing Wu, Yan Lu, Xing-Yan Liu; statistics and draft writing: Guo-Liang Huang, Jin-Hua Qiu, Bin-Bin Li; conception, design and editing: Zhiwei He.

Disclosure of Potential Conflicts of Interest

No potential conflicts of interest were disclosed.

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Affiliations

  1. Sino-American Cancer Research Institute, Guangdong Medical College, No. 1 Xincheng Road, Dongguan, 523808, China

    Guo-Liang Huang, Bin-Bin Li, Jing-Jing Wu, Yan Lu, Xing-Yan Liu & Zhiwei He

  2. Department of Neurology, Huizhou First Hospital, 516000, Huizhou, China

    Jin-Hua Qiu

  3. Key Laboratory for Medical Molecular Diagnostics of Guangdong Province, 523808, Dongguan, China

    Guo-Liang Huang, Bin-Bin Li, Jing-Jing Wu, Yan Lu, Xing-Yan Liu & Zhiwei He

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  1. Guo-Liang Huang
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  2. Jin-Hua Qiu
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  4. Jing-Jing Wu
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  5. Yan Lu
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Corresponding author

Correspondence to Zhiwei He.

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Guo-Liang Huang and Jin-Hua Qiu contributed equally to this study.

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Huang, GL., Qiu, JH., Li, BB. et al. Prolyl Isomerase Pin1 Regulated Signaling Pathway Revealed by Pin1 +/+ and Pin1 −/− Mouse Embryonic Fibroblast Cells. Pathol. Oncol. Res. 19, 667–675 (2013). https://doi.org/10.1007/s12253-013-9629-x

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  • DOI: https://doi.org/10.1007/s12253-013-9629-x

Keywords

  • Pin1
  • Pathway
  • Cancer
  • Immune