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Common Variants at 8q24 are Associated with Prostate Cancer Risk in Serbian Population

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Pathology & Oncology Research

Abstract

Previous studies have shown correlation between single nucleotide polymorphisms (SNPs) at 8q24 and prostate cancer (PCa) risk. This study aimed to evaluate possible association between genotypes and alleles of 8q24 polymorphisms (rs1447295, rs4242382, rs6983267, rs7017300, and rs7837688) and PCa risk and progression. 150 patients with PCa, 150 patients with benign prostatic hyperplasia (BPH), and 100 healthy controls selected from the general population were recruited for this study. SNPs were genotyped by using PCR-RFLP analysis. There was a significant positive association between the A allele of the SNP rs4242382 and PCa risk [PCa vs. BPH comparison, P = 0.014 for the best-fitting dominant model; odds ratio (OR) =1.98; 95 % confidence interval (95%CI) 1.14–3.43]. We found evidence (P = 0.0064) of association between PCa risk and rs7017300 (heterozygote OR = 1.60; 95%CI 0.95–2.69) when comparing genotype distributions in PCa and BPH patients. The association between T allele rs7837688 and PCa risk was determined in PCa vs. BPH comparison with the best-fitting model of inheritance being log-additive (P = 0.0033; OR = 2.14, 95%CI 1.27–3.61). Odds ratio for carriers of rs6983267 TT genotype under recessive model of association with PCa was found to be 0.36 (PCa vs. control comparison, P = 0.0029; 95%CI 0.19–0.71). For rs1447295, deviation from Hardy-Weinberg equilibrium was observed in BPH patients and controls. We found no association between parameters of PCa progression and five 8q24 SNPs. Locus 8q24 harbors genetic variants associated with PCa risk in Serbian population.

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Conflict of Interest

The Authors declare that there is no conflict of interest.

Grant Support

The research was supported by the Ministry of Education and Science of Serbia (Project no. 173016).

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Authors and Affiliations

  1. Faculty of Biology, University of Belgrade, Belgrade, Serbia

    Ana S. Branković, Goran N. Brajušković, Jovan D. Mirčetić, Zorana Z. Nikolić, Predrag B. Kalaba, Dušanka LJ Savić-Pavićević & Stanka P. Romac

  2. Department of Urology, Clinical Centre “dr Dragiša Mišović”, Belgrade, Serbia

    Vinka D. Vukotić

  3. Clinical Department of Surgery, Clinical Centre “Zvezdara”, Belgrade, Serbia

    Saša M. Tomović

  4. Institute of Pathology, Military Medical Academy, Belgrade, Serbia

    Snežana J. Cerović

  5. Faculty of Organizational Science, University of Belgrade, Belgrade, Serbia

    Zoran A. Radojičić

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  1. Ana S. Branković
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  2. Goran N. Brajušković
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  3. Jovan D. Mirčetić
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Corresponding author

Correspondence to Goran N. Brajušković.

Additional information

Branković A. and Brajušković G. contributed equally to this work.

The research was supported by the Ministry of Education and Science of Serbia (Project no. 173016).

Electronic supplementary material

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Supplementary Table S1

The primer sets used for amplification of the regions surrounding selected SNPs at 8q24 (DOC 34 kb)

Supplementary Table S2

PCR amplification profiles (DOC 26 kb)

Supplementary Table S3

Enzyme characteristics and lengths of digested PCR products (DOC 34 kb)

Supplementary Table S4

Association of SNPs rs1447295 and rs4242382 with prostate cancer progression under codominant, dominant and recessive genetic models assessed by the Pearson chi-square test (DOC 79 kb)

Supplementary Table S5

Association of SNPs rs7017300 and rs7837688 with prostate cancer progression under codominant, dominant and recessive genetic models assessed by the Pearson chi-square test (DOC 81 kb)

Supplementary Table S6

Association of the SNP rs6983267 with prostate cancer progression under codominant, dominant and recessive genetic models assessed by the Pearson chi-square test (DOC 64 kb)

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Branković, A.S., Brajušković, G.N., Mirčetić, J.D. et al. Common Variants at 8q24 are Associated with Prostate Cancer Risk in Serbian Population. Pathol. Oncol. Res. 19, 559–569 (2013). https://doi.org/10.1007/s12253-013-9617-1

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