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Human Papillomavirus Types Distribution in Eastern Sicilian Females with cervical lesions. A Correlation with Colposcopic and Histological Findings

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Pathology & Oncology Research

Abstract

To determine human papillomavirus (HPV) types distribution in cervical lesions in a Southern Italian female population in Messina and their relationship between HPV type and grade of colposcopic and histopathological abnormality, a total of 253 women aged 17–68 years, with previous cytological abnormalities, were included in this study. HPV-DNA testing, colposcopy and biopsy were performed. For each sample, cervical cells were collected by centrifugation and DNA was extracted, followed by a PCR-based HPV-DNA assay and reverse dot blot genotyping. HPV-16 was found the most common type (46.6 %) followed by HPV-31 (26.9 %), −6 (18.6 %), −58 (8.8 %), −18 (6.7 %), −66 (5.7 %), −52 and −53 (4.7 %). Out of 62 women with abnormal transformation zone (ATZ) area compatible with squamous intraepithelial lesion (SIL) or cervical cancer (CC), 64.5 % was found high risk (HR) HPV-positive. Moreover the severity of the colposcopic diagnosis was positively correlated with the higher HPV oncogenicity risk (HPV-16 P = 0.023; and HPV-53 P = 0.047). The HPV-16 was found the most prevalent type within each histological category: 66.7 %, 31.2 %, 44 % and 37.2 % of CC, high grade (H)SIL, low grade (L)SIL and chronic cervicitis respectively; followed by HPV-31 present in 25 %, 8 %, and 13.3 % of HSIL, LSIL and chronic cervicitis respectively. A higher HPV incidence than the rest of Italy was found, in agreement with that detected by other authors for the South of the country. These data provide further information about the types prevalence in women with cervical lesions living in Eastern Sicily, suggesting the introduction of new targeted vaccines against a wider spectrum of HPV.

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Authors and Affiliations

  1. Department of Pediatric, Gynecological, Microbiological and Biomedical Sciences, University of Messina, Azienda Ospedaliera Universitaria, ‘Policlinico G. Martino’, Via Consolare Valeria, 98125, Messina, Italy

    Maria Le Donne, Carmela Caruso, Rosalba Scalisi, Benito Chiofalo & Onofrio Triolo

  2. Department of Human Pathology, University of Messina, Azienda Ospedaliera Universitaria, ‘Policlinico G. Martino’, Via Consolare Valeria, 98125, Messina, Italy

    Giuseppe Giuffrè, Piero Antonio Nicotina & Angela Simone

  3. Department of Economical, Business and Environmental Sciences and Quantitative Methods, University of Messina, Via dei Verdi n.75, 98124, Messina, Italy

    Angela Alibrandi

  4. Dipartimento di Scienze Pediatriche, Ginecologiche, Microbiologiche e Biomediche, Azienda Ospedaliera Universitaria, ‘Policlinico G. Martino’, Via Consolare Valeria, 98125, Messina, Italy

    Maria Le Donne

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  1. Maria Le Donne
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Correspondence to Maria Le Donne.

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Le Donne, M., Giuffrè, G., Caruso, C. et al. Human Papillomavirus Types Distribution in Eastern Sicilian Females with cervical lesions. A Correlation with Colposcopic and Histological Findings. Pathol. Oncol. Res. 19, 481–487 (2013). https://doi.org/10.1007/s12253-013-9605-5

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  • DOI: https://doi.org/10.1007/s12253-013-9605-5

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