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Nucleotide Excision Repair Gene Subunit XPD is Highly Expressed in Cervical Squamous Cell Carcinoma

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Pathology & Oncology Research

Abstract

One of the subunits in the mammalian transcription factor II H complex, XPD (TFIIH p80), plays a significant role in the nucleotide excision repair pathway. Events such as abnormal DNA excision repair may be involved in the cervical carcinogenesis process. Expression of the human XPD protein was examined using immunohistochemistry in 84 normal cervical tissues and 148 cervical squamous cell carcinoma samples. Additionally, qRT-PCR was performed to analyse the XPD mRNA expression in 69 fresh normal cervical tissues and 110 cervical carcinoma samples. The relationships between XPD expression and various clinicopathological features (including age, FIGO stage, tumor size, stroma involvement, lymph node metastasis and histologic grade) were assessed. The XPD (TFIIH p80) protein was detected primarily in the cytoplasm. We found a statistically significant difference in XPD expression level in cervical carcinoma versus normal cervical tissue (Z = −7.302, P = 0.000). Notably, XPD mRNA was significantly over-expressed in cervical carcinoma tissues but not in normal cervix tissues (t = 6.942, P = 0.000). However, no statistically significant relationship was found regarding XPD expression and age, FIGO stage, tumor size, stroma involvement, lymph node metastasis or histologic grade (P = 0.089, 0.953, 0.809, 0.275, 0.421, 0.387 respectively). Our results showed that XPD was highly expressed in cervical squamous cell carcinoma tissues. A poorly understood change may occur during the XPD transcription process, resulting in the abnormal enrichment seen from mRNA to the protein level, thus leaving the protein unable to perform the normal function of excision repair. There is a need for further research in order to elucidate the specific mechanism involved.

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Abbreviations

TFIIH:

Transcription factor II H complex

XPD:

Xeroderma pigmentosum D

qRT-PCR:

Quantitative Real-Time PCR

FIGO:

International Federation of Gynecology and Obstetrics

NER:

Nucleotide excision repair

PBS:

Phosphate buffered saline

CIN:

Cervical intraepithelial neoplasia

HPV:

Human papillomavirus

SNP:

Single-nucleotide polymorphism

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Acknowledgments

The authors are grateful to Ms. Minghua Yu for assistance in recruiting the subjects. This work was supported by a grant from the Zhejiang Natural Science Foundation of China (Y204049, Y2110200), from Science and Technology Project of Zhejiang Province, China (No.2005C23003), and from National Nature Science foundation of China (No.30973380).

Author Contribution Statement

FY and HC conceived and carried out experiments and analysed data. JJ conceived experiments and analysed data. CZ and QC carried out experiments. All authors contributed to the writing of the manuscript and had final approval of the submitted and published versions.

Conflict of Interest Statement

The authors have no conflicts of interest to declare.

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Authors and Affiliations

  1. Women’s Reproductive Health Laboratory of Zhejiang Province, Women’s Hospital, School of Medicine, Zhejiang University, Xueshi Rd #2, Hangzhou, 310006, China

    Feng Ye, Qi Cheng & Huaizeng Chen

  2. Department of Gynecological Oncology, Women’s Hospital, School of Medicine, Zhejiang University, Xueshi Rd#2, Hangzhou, 310006, China

    Jie Jiao

  3. Department of Pathology, Women’s Hospital, School of Medicine, Zhejiang University, Xueshi Rd #2, Hangzhou, 310006, China

    Caiyun Zhou

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  1. Feng Ye
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  2. Jie Jiao
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  3. Caiyun Zhou
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  4. Qi Cheng
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  5. Huaizeng Chen
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Correspondence to Huaizeng Chen.

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Ye, F., Jiao, J., Zhou, C. et al. Nucleotide Excision Repair Gene Subunit XPD is Highly Expressed in Cervical Squamous Cell Carcinoma. Pathol. Oncol. Res. 18, 969–975 (2012). https://doi.org/10.1007/s12253-012-9527-7

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