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Dysregulated Expression of Dicer and Drosha in Breast Cancer

Pathology & Oncology Research volume 18, pages 343–348 (2012)Cite this article

Abstract

Large-scale profiling approaches have revealed global down-regulation of microRNAs (miRNAs) in several human cancer types including breast cancer. Altered expression of Dicer and Drosha, two key enzymes in the miRNA maturation, is believed to be one of the most important mechanisms. By using quantitative real-time RT-PCR (QT-PCR), we examined the expression of Dicer and Drosha in 49 pairs of matched human breast cancer tissues. Decreased expression was observed in 53.1% (Dicer), 51.9% (Drosha) and 75.5% (Dicer plus Drosha) breast cancer tissues. In conclusion, the decreased expression of Dicer and Drosha may play a role in down-regulation of miRNAs in breast cancer.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 30901455).

Competing interests

The authors declare that they have no competing interests.

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Affiliations

  1. Department of Oncology, First Affiliated Hospital of Zhengzhou University, No. 1, East Jianshe Rd, Zhengzhou, 450003, People’s Republic of China

    Min Yan & Qing-Xia Fan

  2. Department of Breast Cancer, Affiliated Hospital of Academy of Military Medical Sciences, Beijing, China

    Hong-Yan Huang

  3. Department of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, China

    Ting Wang

  4. Department of Statistics, Fourth Military Medical University, Xi’an, China

    Yi Wan

  5. Breast Cancer Center, Henan Cancer Hospital, Henan, China

    Min Yan, Shu-De Cui & Zhen-zhen Liu

Authors
  1. Min Yan
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  2. Hong-Yan Huang
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  3. Ting Wang
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  4. Yi Wan
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  5. Shu-De Cui
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  6. Zhen-zhen Liu
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  7. Qing-Xia Fan
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Corresponding author

Correspondence to Qing-Xia Fan.

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Min Yan and Hong-Yan Huang contributed Equally

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Yan, M., Huang, HY., Wang, T. et al. Dysregulated Expression of Dicer and Drosha in Breast Cancer. Pathol. Oncol. Res. 18, 343–348 (2012). https://doi.org/10.1007/s12253-011-9450-3

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  • DOI: https://doi.org/10.1007/s12253-011-9450-3

Keywords

  • Breast cancer
  • microRNAs
  • Dicer
  • Drosha