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Study of the Differentially Expressed Genes in Pleomorphic Adenoma Using cDNA Microarrays

Pathology & Oncology Research volume 17, Article number: 765 (2011) Cite this article

Abstract

Recent studies have determined that gene expression profiling using microarray technology can be used to identify tumor-related molecules. The objective of this study was to screen the differentially expressed genes between pleomorphic adenoma (PA) and the normal tissue adjacent to PA using cDNA microarrays and to further validate the differentially expressed genes by real-time PCR. In this study, we selected five pairs of PA and the surrounding normal salivary gland tissues. The total RNA was isolated from tumor and normal tissues and purified to mRNA. The mRNA was reverse-transcribed to cDNA with the incorporation of fluorescent-labeled dUTP to prepare the hybridization probes. The mixed probes were hybridized to Whole Human Gene Expression Microarrays by Agilent. Tumor-related genes were screened by analyzing the fluorescence intensity. As a result, a total of 447 genes were found to be differentially expressed between PA and normal tissue adjacent to PA. Among them, 185 genes were up-regulated and 262 genes were down-regulated in PA. By constructing a network from the differentially expressed genes, some genes, such as Gli2 and CTNNB1, were identified as being at the core of the network. In addition, differential gene expression was validated for 2 up-regulated genes, Gli2 and LOX, using real-time PCR and the results were consistent with those of the cDNA microarray analysis thus verifying the credibility of the microarray data. Therefore, our microarray data may provide clues for finding novel genes involved in the development of PA, and shed light on finding new targets for diagnosis and therapy of PA. Further characterization of these differentially expressed genes will be useful in understanding the genetic basis for PA.

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References

  1. Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25:402–408

    PubMed  Article  CAS  Google Scholar 

  2. Oniscu A, James RM, Morris RG et al (2004) Expression of Sonic hedgehog pathway genes is altered in colonic neoplasia. J Pathol 203:909–917

    PubMed  Article  CAS  Google Scholar 

  3. Dormoy V, Danilin S, Lindner V et al (2009) The sonic hedgehog signaling pathway is reactivated in human renal cell carcinoma and plays orchestral role in tumor growth. Mol Cancer 8:123

    PubMed  Article  Google Scholar 

  4. Katoh Y, Katoh M (2009) Hedgehog target genes: mechanisms of carcinogenesis induced by aberrant hedgehog signaling activation. Curr Mol Med 9:873–886

    PubMed  Article  CAS  Google Scholar 

  5. Ji J, Chen X, Leung SY et al (2002) Comprehensive analysis of the gene expression profiles in human gastric cancer cell lines. Oncogene 21:6549–6556

    PubMed  Article  CAS  Google Scholar 

  6. Sakakura C, Hagiwara A, Nakanishi M et al (2002) Differential gene expression profiles of gastric cancer cells established from primary tumour and malignant ascites. Br J Cancer 87:1153–1161

    PubMed  Article  CAS  Google Scholar 

  7. Kuriakose MA, Chen WT, He ZM et al (2004) Selection and validation of differentially expressed genes in head and neck cancer. CMLS, Cell Mol Life Sci 61:1372–1383

    Article  CAS  Google Scholar 

  8. Balaji KC, Rao PS, Smith DJ et al (2004) Microarray analysis of differential gene expression in androgen independent prostate cancer using a metastatic human prostate cancer cell line model. Urol Oncol 22:313–320

    PubMed  Article  CAS  Google Scholar 

  9. Zhang P, Zhang ZY, Zhou XJ et al (2006) Identification of genes associated with cisplatin resistance in human oral squamous cell carcinoma cell line. BMC Cancer 6:224

    PubMed  Article  Google Scholar 

  10. Guo WF, Lin RX, Huang J et al (2005) Identification of differentially expressed genes contributing to radioresistance in lung cancer cells using microarray analysis. Radiat Res 164:27–35

    PubMed  Article  CAS  Google Scholar 

  11. Kudoh K, Ramanna M, Ravatn R et al (2000) Monitoring the expression profiles of doxorubicin-induced and doxorubicin-resistant cancer cells by cDNA microarray. Cancer Res 60:4161–4166

    PubMed  CAS  Google Scholar 

  12. Kasper M, Regl G, Frischauf AM, Aberger F (2006) GLI transcription factors: mediators of oncogenic Hedgehog signalling. Eur J Cancer 42:437–445

    PubMed  Article  CAS  Google Scholar 

  13. Thiyagarajan S, Bhatia N, Reagan-Shaw S et al (2007) Role of GLI2 transcription factor in growth and tumorigenicity of prostate cells. Cancer Res 67:10642–10646

    PubMed  Article  CAS  Google Scholar 

  14. Snijders AM, Huey B, Connelly ST et al (2009) Stromal control of oncogenic traits expressed in response to the overexpression of GLI2, a pleiotropic oncogene. Oncogene 28:625–637

    PubMed  Article  CAS  Google Scholar 

  15. Varnat F, Duquet A, Malerba M et al (2009) Human colon cancer epithelial cells harbour active HEDGEHOG-GLI signalling that is essential for tumour growth, recurrence, metastasis and stem cell survival and expansion. EMBO Mol Med 1:338–351

    PubMed  Article  CAS  Google Scholar 

  16. Katoh Y, Katoh M (2008) Integrative genomic analyses on GLI2: mechanism of Hedgehog priming through basal GLI2 expression, and interaction map of stem cell signaling network with P53. Int J Oncol 33:881–886

    PubMed  CAS  Google Scholar 

  17. Cheng WT, Tian DY, Xu K (2008) The expression of Gli2 in primary hepatocellular carcinoma and its relationship with Cyclin D1, p21. J Huazhong Univ Sci Technol 37:435–438

    CAS  Google Scholar 

  18. Kim JE, Singh RR, Cho-Vega JH et al (2009) Sonic hedgehog signaling proteins and ATP-binding cassette G2 are aberrantly expressed in diffuse large B-cell lymphoma. Mod Pathol 22:1312–1320

    PubMed  Article  CAS  Google Scholar 

  19. Kim KH, Kim JM, Choi YL et al (2009) Expression of sonic hedgehog signaling molecules in normal, hyperplastic and carcinomatous endometrium. Pathol Int 59:279–287

    PubMed  Article  Google Scholar 

  20. Regl G, Kasper M, Schnidar H, Eichberger T, Neill GW et al (2004) The zinc-finger transcription factor GLI2 antagonizes contact inhibition and differentiation of human epidermal cells. Oncogene 23:1263–1274

    PubMed  Article  CAS  Google Scholar 

  21. Tricarico R, Bet P, Ciambotti B et al (2009) Endometrial cancer and somatic G > T KRAS transversion in patients with constitutional MUTYH biallelic mutations. Cancer Lett 274:266–270

    PubMed  Article  CAS  Google Scholar 

  22. Voz ML, Agten NS, Van de Ven WJ et al (2000) PLAG1, the main translocation target in pleomorphic adenoma of the salivary glands, is a positive regulator of IGF-II. Cancer Res 60:106–113

    PubMed  CAS  Google Scholar 

  23. Van Dyck F, Declercq J, Braem CV et al (2007) PLAG1, the prototype of the PLAG gene family: versatility in tumour development. Int J Oncol 30:765–774

    PubMed  Google Scholar 

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Acknowledgements

This study was supported by grant 08JC1412900 and grant 10DZ1951300 from Science and Technology Commission of Shanghai Municipality, and grant S30206 from Shanghai Leading Academic Discipline Project.

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Affiliations

  1. Department of Stomatology, First People’s Hospital, Shanghai Jiao Tong University School of Medicine, 100 Haining Road, Shanghai, 200080, China

    Meng Song, Cuicui Xiao, Tingle Wang, Qingguo Pei & Shiwei Wang

  2. Department of Oral and Maxillofacial Surgery, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China

    Liqun Xu & Wantao Chen

Authors
  1. Meng Song
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  2. Cuicui Xiao
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  3. Tingle Wang
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  4. Qingguo Pei
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  5. Shiwei Wang
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  6. Liqun Xu
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  7. Wantao Chen
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Correspondence to Meng Song or Wantao Chen.

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Song, M., Xiao, C., Wang, T. et al. Study of the Differentially Expressed Genes in Pleomorphic Adenoma Using cDNA Microarrays. Pathol. Oncol. Res. 17, 765 (2011). https://doi.org/10.1007/s12253-011-9384-9

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  • DOI: https://doi.org/10.1007/s12253-011-9384-9

Keywords

  • cDNA microarray
  • Differentially expression genes
  • Gene chip
  • Pleomorphic adenoma