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Stepwise Positive Association Between APOA5 Minor Allele Frequencies and Increasing Plasma Triglyceride Quartiles in Random Patients with Hypertriglyceridemia of Unclarified Origin

  • Published:
Pathology & Oncology Research

Abstract

Apolipoprotein A5 (ApoA5) gene and its protein product play a central role in the complex regulation of circulating triglyceride levels in humans. Naturally occurring variants of the apolipoprotein A5 gene have been associated with increased triglyceride levels and have been found to confer risk for cardiovascular diseases. In our study, four polymorphisms, the T-1131C, IVS3+G476A, T1259C, and C56G alleles of APOA5 were analyzed in a total of 436 patients by polymerase chain reaction—restriction fragment length polymorphism methods. The randomly selected patients were classified into four quartile (q) groups based on triglyceride levels (q1: TG<1.31 mmol/l; q2: 1.31–2.90 mmol/l; q3: 2.91–4.85 mmol/l; q4: TG>4.85 mmol/l). We observed significant stepwise increasing association between the four APOA5 minor allele carrier frequencies and plasma triglyceride quartiles: -1131C (q1: 4.44%; q2: 8.95%; q3: 12.9%; q4: 20.6%), IVS3+476A (q1: 4.44%; q2: 5.79%; q3: 11.1%; q4: 19.7%), 1259C (q1: 4.44%; q2: 6.84%; q3: 11.1%; q4: 20.6%) and 56G (q1: 5.64%; q2: 6.31%; q3: 11.16%; q4: 11.9%). The serum total cholesterol and high density lipoprotein-cholesterol levels also showed allele-dependent differences in the quartiles. The findings presented here revealed a special arrangement of APOA5 minor alleles in patients with different serum triglyceride ranges in Hungarians.

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Abbreviations

APOA5:

Apolipoprotein A5

HTG:

Hypertrigyceridaemia

LPL:

Lipoprotein lipase

SNP:

Single nucleotide polymorphism

VLDL:

Very-low-density lipoprotein

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Acknowledgement

This work was supported by the grant of Hungarian Scientific Research Foundation OTKA T73430 and ETT 243 /2009.

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Authors and Affiliations

  1. Central Laboratory, Markusovszky County Hospital, Szombathely, Hungary

    Ferenc Hadarits

  2. Department of Medical Genetics, University of Pécs, Pécs, Hungary

    Péter Kisfali, Anita Maász, Katalin Sümegi, Andrea Valasek, Ingrid Janicsek & Béla Melegh

  3. 2nd Department of Medicine and Nephrological Center, University of Pécs, Pécs, Hungary

    Márton Mohás & István Wittmann

  4. Department of Pulmonology, Robert Koch Hospital, Edelény, Hungary

    Melinda Szabó

  5. Central Laboratory, Aladar Petz County Hospital, Győr, Hungary

    Katalin Hetyésy

  6. Department of Medical Genetics and Child Development, University of Pécs, Pécs, Szigeti 12, 7624, Hungary

    Béla Melegh

Authors
  1. Ferenc Hadarits
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  2. Péter Kisfali
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Correspondence to Béla Melegh.

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Hadarits, F., Kisfali, P., Mohás, M. et al. Stepwise Positive Association Between APOA5 Minor Allele Frequencies and Increasing Plasma Triglyceride Quartiles in Random Patients with Hypertriglyceridemia of Unclarified Origin. Pathol. Oncol. Res. 17, 39–44 (2011). https://doi.org/10.1007/s12253-010-9273-7

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  • DOI: https://doi.org/10.1007/s12253-010-9273-7

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