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The Plasminogen System in Microdissected Colonic Mucosa Distant from an Isolated Adenoma

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Pathology & Oncology Research

Abstract

In the colon, the urokinase-type plasminogen activator (uPA), its receptor (uPAR), and plasminogen activator inhibitors, PAI-1 and PAI-2, are implicated in the transition from mucosa to adenoma and tumour progression. However, expression in the mucosa adjacent, or distant, to an adenoma has not yet been investigated. Three biopsies from mucosae adjacent (20 cm, ipsilateral) and distant (contralateral) to an isolated tubular adenoma were analysed in 14 patients and 8 controls. Laser microdissection isolated stromal and epithelial crypt components, and quantitative RT-PCR analyses of uPA, uPAR, PAI-1 and PAI-2 mRNA levels were performed. Among controls, no significant differences in the markers were noted. With left colon isolated tubular adenoma, uPA, uPAR, and PAI-2 mRNA levels were significantly increased in the adjacent mucosal stroma compared to epithelial crypt levels (p < 0.05). In right colon adenoma, the mRNA levels of these 3 molecular markers were significantly increased only in the adjacent mucosal stromal samples (p < 0.05). Isolated tubular adenoma in the colon increases significantly the mRNA levels of 3 proteolysis-associated molecular markers in the stromal, but not in the epithelial, components of adjacent mucosa. These results suggest the presence of regional and dynamic interactions in apparently non-involved mucosae.

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Authors and Affiliations

  1. Division of Gastroenterology and Hepatology, University Hospital Lausanne, Lausanne, 1011, Switzerland

    Francois Saucy, Daniel Bachmann & Gian Dorta

  2. Swiss Institute for Experimental Cancer Research, Epalinges, 1066, Switzerland

    Olivier Peterman, Bernard Sordat & Isabelle Sordat

  3. Centre Hospitalier Universitaire Vaudois, Lausanne, 1011, Switzerland

    Francois Saucy

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  1. Francois Saucy
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  2. Daniel Bachmann
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  3. Olivier Peterman
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  4. Bernard Sordat
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  5. Isabelle Sordat
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  6. Gian Dorta
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Correspondence to Francois Saucy.

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Saucy, F., Bachmann, D., Peterman, O. et al. The Plasminogen System in Microdissected Colonic Mucosa Distant from an Isolated Adenoma. Pathol. Oncol. Res. 17, 25–31 (2011). https://doi.org/10.1007/s12253-010-9268-4

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  • DOI: https://doi.org/10.1007/s12253-010-9268-4

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