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Quantification of Dendritic Cells and Osteoclasts in the Bone Marrow of Patients with Monoclonal Gammopathy

  • Original Paper
  • Published:
Pathology & Oncology Research

Abstract

The purpose of this study was to find histological clues for reliable differentiation between monoclonal gammopathy of undetermined significance (MGUS) and myeloma when clinical parameters are controversial. Differential appearance of dendritic cells and osteoclasts, two cell types developing from the monocytic lineage upon distinct cytokine activation profile, might be a useful approach. Bone and bone-marrow biopsies performed in 105 patients were studied using histomorphometry after identification of osteoclasts (by histochemical identification of tartrate resistant acid phosphatase) and dendritic cells (by immunohistochemical detection of the S-100 protein). Patients were classified by the World Health Organization criteria but histopathological criteria were more adapted to identify MGUS (53 cases), myeloma (46), B-cell lymphoma (six) since six myeloma were not correctly classified. Histomorphometry was compared to 15 control cases. The number of marrow dendritic cell was significantly increased with B-cell lymphoma >MGUS >myeloma > controls. Dendritic cell were often mixed with lymphoma cells. Myeloma had increased bone resorption with a high osteoclast number and moderate increase in dendritic cells. B-cell lymphomas had a considerable increase in dendritic cell but presented mononucleated osteoclasts. These findings can help in the classification of MGUS in the early stages of the disease and could help to propose preventive treatments.

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Abbreviations

BL:

B lymphoma

BV/TV:

trabecular bone volume

CFU-M:

colony forming unit (monocytic)

DC:

dendritic cell

ES/BS:

Eroded surfaces

IL-6:

interleukin 6

M-CSF:

mononuclear phagocyte colony-stimulating factor

MGUS:

monoclonal gammopathy of undetermined significance

MM:

multiple myeloma

NOc/B.Ar:

number of osteoclasts (bone referent)

NOc/T.Ar:

number of osteoclasts (tissue referent)

Oc:

osteoclast

OPG:

osteoprotegerin

OS/BS:

osteoid surfaces

OV/BV:

osteoid volume

RANK:

receptor activator of nuclear factor-κ B

RANKL:

receptor activator of nuclear factor-κ B ligand

TRAcP:

tartrate-resistant acid phosphatase

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Acknowledgments

This work was supported in part by financial help of INSERM. Authors have no conflict of interest.

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Authors and Affiliations

  1. Faculté de Médecine, INSERM, U922-LHEA, 49045, Angers Cédex, France

    Nicolas Josselin, Hélène Libouban, Erick Legrand, Michel Félix Baslé, Maurice Audran & Daniel Chappard

  2. Service d’hématologie and UPRES EA 3863, CHU d’Angers, 49933, Angers Cédex, France

    Mamoun Dib & Norbert Ifrah

  3. Service de Rhumatologie, CHU d’Angers, 49933, Angers Cédex, France

    Erick Legrand & Maurice Audran

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  1. Nicolas Josselin
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  2. Hélène Libouban
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  3. Mamoun Dib
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  8. Daniel Chappard
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Correspondence to Daniel Chappard.

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Josselin, N., Libouban, H., Dib, M. et al. Quantification of Dendritic Cells and Osteoclasts in the Bone Marrow of Patients with Monoclonal Gammopathy. Pathol. Oncol. Res. 15, 65–72 (2009). https://doi.org/10.1007/s12253-008-9092-2

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  • DOI: https://doi.org/10.1007/s12253-008-9092-2

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