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Development and Characterization of SYBR Green I Based RT-PCR Assay for Detection of Omsk Hemorrhagic Fever Virus

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Virologica Sinica

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References

  • Casals J (1961) Procedures for identification of arthropod-borne viruses. Bull World Health Organ 24:723–734

    CAS  PubMed  PubMed Central  Google Scholar 

  • Chambers TJ, Hahn CS, Galler R, Rice CM (1990) Flavivirus genome organization, expression, and replication. Annu Rev Microbiol 44:649–688

    Article  CAS  Google Scholar 

  • Dobler G (2010) Zoonotic tick-borne flaviviruses. Vet Microbiol 140:221–228

    Article  Google Scholar 

  • Hanaki K, Ike F, Kajita A, Yasuno W, Yanagiba M, Goto M, Sakai K, Ami Y, Kyuwa S (2014) A broadly reactive one-step SYBR Green I real-time RT-PCR assay for rapid detection of murine norovirus. PLoS ONE 9:e98108

    Article  Google Scholar 

  • Ho PS, Ng MM, Chu JJ (2010) Establishment of one-step SYBR green-based real time-PCR assay for rapid detection and quantification of chikungunya virus infection. Virol J 7:13

    Article  Google Scholar 

  • Holbrook MR, Aronson JF, Campbell GA, Jones S, Feldmann H, Barrett AD (2005) An animal model for the tickborne flavivirus–Omsk hemorrhagic fever virus. J Infect Dis 191:100–108

    Article  Google Scholar 

  • Jelinkova-Skalova E, Tesarova J, Buresova D, Kouba K, Hronovsky V (1974) Laboratory infection with virus of Omsk haemorrhagic fever with neurological and psychiatric symptomatology (author’s transl). Cesk Epidemiol Mikrobiol Imunol 23:290–293 (In Czech)

    CAS  PubMed  Google Scholar 

  • Kong LL, Omar AR, Hair Bejo M, Ideris A, Tan SW (2009) Development of SYBR green I based one-step real-time RT-PCR assay for the detection and differentiation of very virulent and classical strains of infectious bursal disease virus. J Virol Methods 161:271–279

    Article  CAS  Google Scholar 

  • Lani R, Moghaddam E, Haghani A, Chang LY, AbuBakar S, Zandi K (2014) Tick-borne viruses: a review from the perspective of therapeutic approaches. Ticks Tick Borne Dis 5:457–465

    Article  Google Scholar 

  • Maher-Sturgess SL, Forrester NL, Wayper PJ, Gould EA, Hall RA, Barnard RT, Gibbs MJ (2008) Universal primers that amplify RNA from all three flavivirus subgroups. Virol J 5:16

    Article  Google Scholar 

  • Porterfield JS (1957) Use of goose cells in haemagglutination tests with arthropod-borne viruses. Nature 180:1201–1202

    Article  CAS  Google Scholar 

  • Ruzek D, Yakimenko VV, Karan LS, Tkachev SE (2010) Omsk haemorrhagic fever. Lancet 376:2104–2113

    Article  Google Scholar 

  • Yoshii K, Holbrook MR (2009) Sub-genomic replicon and virus-like particles of Omsk hemorrhagic fever virus. Arch Virol 154:573–580

    Article  CAS  Google Scholar 

  • Zhang Q, Li N, Deng C, Zhang Z, Li X, Yoshii K, Ye H, Zhang B (2019) Trans complementation of replication-defective omsk hemorrhagic fever virus for antiviral study. Virol Sin 34:412–422

    Article  CAS  Google Scholar 

  • Zhu Z, Fan H, Qi X, Qi Y, Shi Z, Wang H, Cui L, Zhou M (2013) Development and evaluation of a SYBR green-based real time RT-PCR assay for detection of the emerging avian influenza A (H7N9) virus. PLoS ONE 8:e80028

    Article  Google Scholar 

Download references

Acknowledgements

This work was supported by the National Major Science and Technology Project (2018ZX10711001-006), and the Wuhan Science and Technology Project (2018201261638501).

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Authors and Affiliations

  1. Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, 430071, China

    Ya-Nan Zhang, Cheng-Lin Deng, Zhi-Ming Yuan, Bo Zhang & Han-Qing Ye

  2. Department of Medical Genetics, Oslo University Hospital, 0450, Oslo, Norway

    Si-Qing Liu

  3. School of Medicine, Hunan Normal University, Changsha, 410081, China

    Xiao-Dan Li

Authors
  1. Ya-Nan Zhang
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  2. Si-Qing Liu
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  3. Cheng-Lin Deng
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  4. Zhi-Ming Yuan
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  5. Bo Zhang
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  6. Xiao-Dan Li
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  7. Han-Qing Ye
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Corresponding authors

Correspondence to Xiao-Dan Li or Han-Qing Ye.

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This article does not contain any studies with human or animal subjects performed by any of the authors.

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Zhang, YN., Liu, SQ., Deng, CL. et al. Development and Characterization of SYBR Green I Based RT-PCR Assay for Detection of Omsk Hemorrhagic Fever Virus. Virol. Sin. 36, 1644–1647 (2021). https://doi.org/10.1007/s12250-021-00389-5

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  • DOI: https://doi.org/10.1007/s12250-021-00389-5

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