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Human Cytomegalovirus Influences Host circRNA Transcriptions during Productive Infection

  • RESEARCH ARTICLE
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Virologica Sinica

A Correction to this article was published on 10 November 2020

This article has been updated

Abstract

Human cytomegalovirus (HCMV) is a double-strand DNA virus widely infected in human. Circular RNAs (circRNAs) are non-coding RNAs with most functions of which keep unknown, and the effects of HCMV productive infection on host circRNA transcriptions remain unclear. In this study, we profiled 283 host circRNAs that significantly altered by HCMV productive infection in human embryonic lung fibroblasts (HELF) by RNA deep sequencing and bioinformatics analysis. Among these, circSP100, circMAP3K1, circPLEKHM1, and circTRIO were validated for their transcriptions and sequences. Furthermore, characteristics of circSP100 were investigated by RT-qPCR and northern blot. It was implied that circSP100 was produced from the sense strand of the SP100 gene containing six exons. Kinetics of circSP100 and SP100 mRNA were significantly different after infection: circSP100 levels increased gradually along with infection, whereas SP100 mRNA levels increased in the beginning and dropped at 24 h post-infection (hpi). Meanwhile, a total number of 257 proteins, including 10 HCMV encoding proteins, were identified potentially binding to cytoplasmic circSP100 by RNA antisense purification (RAP) and mass spectrometry. Enrichment analysis showed these proteins were mainly involved in the spliceosome, protein processing, ribosome, and phagosome pathways, suggesting multiple functions of circSP100 during HCMV infection.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (81672028 and 81371788).

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Authors and Affiliations

  1. Virology Laboratory, The Affiliated Shengjing Hospital, China Medical University, Shenyang, 110004, China

    Jingui Deng, Yujing Huang, Qing Wang, Jianming Li, Yanping Ma, Ying Qi, Zhongyang Liu & Qiang Ruan

  2. Department of Laboratory, Central Hospital Affiliated to Shenyang Medical College, Shenyang, 110024, China

    Jingui Deng

  3. Department of Gynecology and Obstetrics, The Affiliated Shengjing Hospital, China Medical University, Shenyang, 110004, China

    Yibo Li

  4. Department of Obstetrics, Central Hospital Affiliated to Shenyang Medical College, Shenyang, 110024, China

    Yibo Li

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  1. Jingui Deng
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  2. Yujing Huang
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  3. Qing Wang
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  5. Yanping Ma
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Contributions

JD, YH and QR conceived and designed the experiments. JD, QW, JL, YL and ZL performed the experiments. YM and YQ analyzed the data. JD and YH wrote the manuscript and prepared the figures. YM and YQ provided helpful suggestions about the study. QR checked and finalized the manuscript. All authors read and approved the final manuscript.

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Correspondence to Yujing Huang or Qiang Ruan.

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Deng, J., Huang, Y., Wang, Q. et al. Human Cytomegalovirus Influences Host circRNA Transcriptions during Productive Infection. Virol. Sin. 36, 241–253 (2021). https://doi.org/10.1007/s12250-020-00275-6

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