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Cha SY, Roh JH, Kang M, Kim B, Jang HK (2013) Isolation and characterization of a low pathogenic duck hepatitis A virus 3 from South Korea. Vet Microbiol 162:254–258
Chen LL, Xu Q, Zhang RH, Yang L, Li JX, Xie ZJ, Zhu YL, Jiang SJ, Si XK (2013) Improved duplex RT-PCR assay for differential diagnosis of mixed infection of duck hepatitis A virus type 1 and type 3 in ducklings. J Virol Methods 192:12–17
Chen X, Chen Y, Liu C, Li X, Liu H, Yin X, Bai X, Ge M, Chen H, Liu M, Du Y, Fan G, Zhang Y (2019) Improved one-tube RT-PCR method for simultaneous detection and genotyping of duck hepatitis A virus subtypes 1 and 3. PLoS ONE 14:e0219750
Ding C, Zhang D (2007) Molecular analysis of duck hepatitis virus type 1. Virology 361:9–17
Doan HT, Le XT, Do RT, Hoang CT, Nguyen KT, Le TH (2016) Molecular genotyping of duck hepatitis A viruses (DHAV) in Vietnam. J Infect Dev Ctries 10:988–995
Gan Y, Huang C, Wang X, Chen S, Li J (2014) Epidemiological investigation of duck hepatitis A virus (DHAV) isolated from Sichuan Basin by RT-PCR disclose the existence of mixed infection and the feasibility of DHAV-A evolved from C80 strain. Pak Vet J 34:356–360
Hu Q, Zhu D, Ma G, Cheng A, Wang M, Chen S, Jia R, Liu M, Sun K, Yang Q (2016) A one-step duplex rRT-PCR assay for the simultaneous detection of duck hepatitis A virus genotypes 1 and 3. J Virol Methods 236:207–214
Huang Q, Yue H, Zhang B, Nie P, Tang C (2012) Development of a real-time quantitative PCR for detecting duck hepatitis a virus genotype C. J Clin Microbiol 50:3318–3323
Kim MC, Kwon YK, Joh SJ, Lindberg AM, Kwon JH, Kim JH, Kim SJ (2006) Molecular analysis of duck hepatitis virus type 1 reveals a novel lineage close to the genus Parechovirus in the family Picornaviridae. J Gen Virol 87:3307–3316
Kim MC, Kwon YK, Joh SJ, Kim SJ, Tolf C, Kim JH, Sung HW, Lindberg AM, Kwon JH (2007) Recent Korean isolates of duck hepatitis virus reveal the presence of a new geno- and serotype when compared to duck hepatitis virus type 1 type strains. Arch Virol 152:2059–2072
Lin SL, Cong RC, Zhang RH, Chen JH, Xia LL, Xie ZJ, Wang Y, Zhu YL, Jiang SJ (2016) Circulation and in vivo distribution of duck hepatitis A virus types 1 and 3 in infected ducklings. Arch Virol 161:405–416
Liu M, Fanyi M, Li XJ, Zhang Z, Liu S, Zhang Y (2011) Goose haemorrhagic hepatitis caused by a new subtype duck hepatitis type 1 virus. Vet Microbiol 152:280–283
Soliman M, Alfajaro MM, Lee MH, Jeong YJ, Kim DS, Son KY, Kwon J, Choi JS, Lim JS, Choi JS, Lee TU, Cho KO, Kang MI (2015) The prevalence of duck hepatitis A virus types 1 and 3 on Korean duck farms. Arch Virol 160:493–498
Toth TE (1969) Studies of an agent causing mortality among ducklings immune to duck virus hepatitis. Avian Dis 13:834–846
Tseng CH, Knowles NJ, Tsai HJ (2007) Molecular analysis of duck hepatitis virus type 1 indicates that it should be assigned to a new genus. Virus Res 123:190–203
Tseng CH, Tsai HJ (2007) Molecular characterization of a new serotype of duck hepatitis virus. Virus Res 126:19–31
Wen X, Zhu D, Cheng A, Wang M, Chen S, Jia R, Liu M, Sun K, Zhao X, Yang Q, Wu Y, Chen X (2018) Molecular epidemiology of duck hepatitis a virus types 1 and 3 in China, 2010–2015. Transbound Emerg Dis 65:10–15
Yang M, Cheng A, Wang M, Xing H (2008) Development and application of a one-step real-time Taqman RT-PCR assay for detection of Duck hepatitis virus type1. J Virol Methods 153:55–60
Yugo DM, Hauck R, Shivaprasad HL, Meng XJ (2016) Hepatitis virus infections in poultry. Avian Dis 60:576–588
Zhang R, Xia L, Chen J, Gong Y, Zhang L, Li P, Liu H, Xie Z, Jiang S (2017) Molecular epidemiology and genetic diversity of duck hepatitis A virus type 3 in Shandong province of China, 2012–2014. Acta Virol 61:463–472
This work was supported by the National Natural Science Foundation of China Grants 31300141 (to CCM) and Shanghai Key Laboratory of Veterinary Biotechnology Grants klab201702 (to CCM).
Conflict of interest
The authors declare that they have no conflict of interest.
Animal and Human Rights Statement
This research was approved by the Ethics Committee of Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences. The international guidelines for the care and use of animals have been followed.
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Meng, C., Huang, Y., Rehman, Z.U. et al. Development of an MCA-Based Real Time RT-qPCR Assay for the Simultaneous Detection and Differentiation of Duck Hepatitis A Virus Types 1 and 3. Virol. Sin. 35, 666–669 (2020). https://doi.org/10.1007/s12250-020-00211-8