Screening and Identification of Marburg Virus Entry Inhibitors Using Approved Drugs

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  1. Bai JPF, Hsu CW (2018) Drug repurposing for Ebola virus disease: principles of consideration and the animal rule. J Pharm Sci 108:798–806

    Article  Google Scholar 

  2. Bixler SL, Bocan TM, Wells J, Wetzel KS, Van Tongeren SA, Dong L, Garza NL, Donnelly G, Cazares LH, Nuss J, Soloveva V, Koistinen KA, Welch L, Epstein C, Liang LF, Giesing D, Lenk R, Bavari S, Warren TK (2017) Efficacy of favipiravir (T-705) in nonhuman primates infected with Ebola virus or Marburg virus. Antivir Res 151:97–104

    Article  Google Scholar 

  3. Brauburger K, Hume AJ, Muhlberger E, Olejnik J (2012) Forty-five years of Marburg virus research. Viruses 4:1878–1927

    Article  Google Scholar 

  4. Cross RW, Mire CE, Feldmann H, Geisbert TW (2018) Post-exposure treatments for Ebola and Marburg virus infections. Nat Rev Drug Discov 17:413–434

    CAS  Article  Google Scholar 

  5. Flyak AI, Ilinykh PA, Murin CD, Garron T, Shen X, Fusco ML, Hashiguchi T, Bornholdt ZA, Slaughter JC, Sapparapu G, Klages C, Ksiazek TG, Ward AB, Saphire EO, Bukreyev A, Crowe JE Jr (2015) Mechanism of human antibody-mediated neutralization of Marburg virus. Cell 160:893–903

    CAS  Article  Google Scholar 

  6. Flyak AI, Shen X, Murin CD, Turner HL, David JA, Fusco ML, Lampley R, Kose N, Ilinykh PA, Kuzmina N, Branchizio A, King H, Brown L, Bryan C, Davidson E, Doranz BJ, Slaughter JC, Sapparapu G, Klages C, Ksiazek TG, Saphire EO, Ward AB, Bukreyev A, Crowe JE Jr (2016) Cross-reactive and potent neutralizing antibody responses in human survivors of natural Ebola virus infection. Cell 164:392–405

    CAS  Article  Google Scholar 

  7. Kouznetsova J, Sun W, Martinez-Romero C, Tawa G, Shinn P, Chen CZ, Schimmer A, Sanderson P, McKew JC, Zheng W, Garcia-Sastre A (2014) Identification of 53 compounds that block Ebola virus-like particle entry via a repurposing screen of approved drugs. Emerg Microbes Infect 3:e84

    CAS  Article  Google Scholar 

  8. Leffel EK, Reed DS (2004) Marburg and Ebola viruses as aerosol threats. Biosecur Bioterror 2:186–191

    Article  Google Scholar 

  9. Madrid PB, Chopra S, Manger ID, Gilfillan L, Keepers TR, Shurtleff AC, Green CE, Iyer LV, Dilks HH, Davey RA, Kolokoltsov AA, Carrion R Jr, Patterson JL, Bavari S, Panchal RG, Warren TK, Wells JB, Moos WH, Burke RL, Tanga MJ (2013) A systematic screen of FDA-approved drugs for inhibitors of biological threat agents. PLoS ONE 8:e60579

    CAS  Article  Google Scholar 

  10. Madrid PB, Panchal RG, Warren TK, Shurtleff AC, Endsley AN, Green CE, Kolokoltsov A, Davey R, Manger ID, Gilfillan L, Bavari S, Tanga MJ (2015) Evaluation of Ebola virus inhibitors for drug repurposing. ACS Infect Dis 1:317–326

    CAS  Article  Google Scholar 

  11. Marzi A, Banadyga L, Haddock E, Thomas T, Shen K, Horne EJ, Scott DP, Feldmann H, Ebihara H (2016) A hamster model for Marburg virus infection accurately recapitulates Marburg hemorrhagic fever. Sci Rep 6:39214

    CAS  Article  Google Scholar 

  12. Wang J, Cheng H, Ratia K, Varhegyi E, Hendrickson WG, Li J, Rong L (2014) A comparative high-throughput screening protocol to identify entry inhibitors of enveloped viruses. J Biomol Screen 19:100–107

    CAS  Article  Google Scholar 

  13. Zhang L, Li Q, Liu Q, Huang W, Nie J, Wang Y (2017) A bioluminescent imaging mouse model for Marburg virus based on a pseudovirus system. Hum Vaccin Immunother 13:1811–1817

    Article  Google Scholar 

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We are grateful to Zongge Zhao and Lihua Xie from the Institute for Reference Standards and Standardization, National Institutes for Food and Drug Control, for providing the compounds. This work was supported by the National Key Research and Development Plan of China (Grant No. 2016YFC1200904). The funding bodies had no role in the study design, data collection or analysis, decision to publish, or preparation of the manuscript.

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Correspondence to Youchun Wang.

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All institutional and national guidelines for the care and use of laboratory animals were followed.

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Zhang, L., Lei, S., Xie, H. et al. Screening and Identification of Marburg Virus Entry Inhibitors Using Approved Drugs. Virol. Sin. 35, 235–239 (2020).

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