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The Limitation of Rapid Tests for DENV2 Infection in Host with Unique Immune Status: Low NS1 Antigenemia and Deficient Antibody Responses

Virologica Sinica volume 35, pages 478–480 (2020)Cite this article

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References

  • Alcon S, Talarmin A, Debruyne M, Falconar A, Deubel V, Flamand M (2002) Enzyme-linked immunosorbent assay specific to Dengue virus type 1 nonstructural protein NS1 reveals circulation of the antigen in the blood during the acute phase of disease in patients experiencing primary or secondary infections. J Clin Microbiol 40:376–381

    Article  CAS  Google Scholar 

  • Bosch I, Puig H, Hiley M, Carré-Camps M, Perdomo Celis F, Narváez C, Salgado D, Senthoor D, Grady M, Phillips E, Durbin A, Fandos D, Miyazaki H, Yen C W, Gélvez-Ramírez M, Warke R, Ribeiro L, Teixeira M, Almeida R, Marques E (2017) Rapid antigen tests for dengue virus serotypes and Zika virus in patient serum. Sci Transl Med 9:1589–1601

    Article  CAS  Google Scholar 

  • Lai S, Huang Z, Zhou H, Anders KL, Perkins TA, Yin W, Li Y, Mu D, Chen Q, Zhang Z, Qiu Y, Wang L, Zhang H, Zeng L, Ren X, Geng M, Li Z, Tatem AJ, Hay SI, Yu H (2015) The changing epidemiology of dengue in China, 1990–2014: a descriptive analysis of 25 years of nationwide surveillance data. BMC Med 13:100–111

    Article  Google Scholar 

  • Le Huynh Thi D, Ngoc T, Ha D, Vu T, Kieu N, Young P, Farrar J, Simmons C, Wolbers M, Wills B (2011) Kinetics of plasma viremia and soluble nonstructural protein 1 concentrations in dengue: differential effects according to serotype and immune status. J Infect Dis 203:1292–1300

    Article  CAS  Google Scholar 

  • Luo L, Jiang L-Y, Xiao X-C, Di B, Jing Q-L, Wang S-Y, Tang J-L, Wang M, Tang X-P, Yang Z-C (2017) The dengue preface to endemic in mainland China: the historical largest outbreak by Aedes albopictus in Guangzhou, 2014. Infect Dis Poverty 6:148–158

    Article  Google Scholar 

  • Nguyen THT, Clapham HE, Phung KL, Nguyen TK, Dinh TT, Nguyen THQ, Tran VN, Whitehead S, Simmons C, Wolbers M, Wills B (2018) Methods to discriminate primary from secondary dengue during acute symptomatic infection. BMC Infect Dis 18:375–384

    Article  CAS  Google Scholar 

  • Pang JV, Chia PY, Lye D, Leo Y (2017) Progress and challenges towards point-of-care diagnostic development for dengue. J Clin Microbiol 55:707–717

    Article  Google Scholar 

  • Prince H, Yeh C, Lapé-Nixon M (2011) Utility of IgM/IgG ratio and IgG avidity for distinguishing primary and secondary dengue virus infections using sera collected more than 30 days after disease onset. Clin Vaccine Immunol 18:1951–1956

    Article  CAS  Google Scholar 

  • Shan X, Wang X, Yuan Q, Zheng Y, Zhang H, Wu Y, Yang J (2015) Evaluation of the diagnostic accuracy of nonstructural protein 1 Ag-based tests for dengue virus in Asian population: a meta-analysis. BMC Infect Dis 15:360–367

    Article  CAS  Google Scholar 

  • Tuekprakhon A, Nakayama E, Bartholomeeusen K, Puiprom O, Sasaki T, Huits R, Luplertlop N, Kosoltanapiwat N, Maneekan P, Ariën K, Shioda T, Leaungwutiwong P (2018) Variation at position 350 in the Chikungunya virus 6K-E1 protein determines the sensitivity of detection in a rapid E1-antigen test. Sci Rep 8:1094–1105

    Article  CAS  Google Scholar 

  • Watanabe S, Tan KH, Rathore A, Rozen-Gagnon K, Shuai W, Ruedl C, Vasudevan S (2012) The magnitude of dengue virus NS1 protein secretion is strain dependent and does not correlate with severe pathologies in the mouse infection model. J Virol 86:5508–5514

    Article  CAS  Google Scholar 

  • Zhao H, Zhao L, Jiang T, Li X-F, Fan H, Hong W, Zhang Y, Zhu Q, Ye Q, Tong Y, Cao W-C, Zhang F, Qin C-F (2014) Isolation and characterization of dengue virus serotype 2 from the large dengue outbreak in Guangdong, China in 2014. Sci China Life Sci 57:1149–1155

    Article  CAS  Google Scholar 

  • Zhao H, Zhang F-C, Zhu Q, Wang J, Hong W-X, Zhao L-Z, Deng Y-Q, Qiu S, Zhang Y, Cai W-P, Cao W-C, Qin C-F (2016) Epidemiological and virological characterizations of the 2014 dengue outbreak in Guangzhou, China. PLoS ONE 11:548–557

    Google Scholar 

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Acknowledgements

We thank Dr. Yongjun Guan for the consulting of the capture ELISA and the isolation of antibody. This Project was supported by funds from 2017 Guangzhou major projects of collaborative innovation in health care (201704020229), the State Key Laboratory of Pathogen and Biosecurity (SKLPBS1839).

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Author notes

  1. Lei Yu and Yingfen Wen contributed equally to this work.

Authors and Affiliations

  1. Guangzhou Eighth People’s Hospital, Guangzhou Medical University, Guangzhou, 510060, China

    Lei Yu, Yingfen Wen, Mengrong Xiang, Wenxin Hong, Lingzhai Zhao & Fuchun Zhang

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  1. Lei Yu
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  2. Yingfen Wen
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  4. Wenxin Hong
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Correspondence to Lei Yu.

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The authors declare that they have no competing interests.

Informed Consent

The study was approved by the Ethical Committee of the Guangzhou Eighth People’s Hospital (20131224). The samples were collected and used in strict accordance with relevant guidelines and regulations.

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Yu, L., Wen, Y., Xiang, M. et al. The Limitation of Rapid Tests for DENV2 Infection in Host with Unique Immune Status: Low NS1 Antigenemia and Deficient Antibody Responses. Virol. Sin. 35, 478–480 (2020). https://doi.org/10.1007/s12250-019-00183-4

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  • DOI: https://doi.org/10.1007/s12250-019-00183-4