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Autographa Californica Multiple Nucleopolyhedrovirus P48 (Ac103) Is Required for the Efficient Formation of Virus-Induced Intranuclear Microvesicles

Virologica Sinica volume 34, pages 712–721 (2019)Cite this article

Abstract

Our previous study has shown that the Autographa californica multiple nucleopolyhedrovirus (AcMNPV) p48 (ac103) gene is essential for the nuclear egress of nucleocapsids and the formation of occlusion-derived virions (ODVs). However, the exact role of p48 in the morphogenesis of ODVs remains unknown. In this study, we demonstrated that p48 was required for the efficient formation of intranuclear microvesicles. To further understand its functional role in intranuclear microvesicle formation, we characterized the distribution of the P48 protein, which was found to be associated with the nucleocapsid and envelope fractions of both budded virions and ODVs. In AcMNPV-infected cells, P48 was predominantly localized to nucleocapsids in the virogenic stroma and the nucleocapsids enveloped in ODVs, with a limited but discernible distribution in the plasma membrane, nuclear envelope, intranuclear microvesicles, and ODV envelope. Furthermore, coimmunoprecipitation assays showed that among the viral proteins required for intranuclear microvesicle formation, P48 associated with Ac93 in the absence of viral infection.

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Acknowledgements

We thank Prof. Zhihong Hu (Wuhan Institute of Virology) for the generous gift of E25 polyclonal antiserum. This research was supported by the National Natural Science Foundation of China (31572056 and 31872025), the Key Project of Natural Science Foundation of Guangdong Province (2018B030311018), the National Key R&D Program of China (2017YFD0200404), and the Guangzhou Science and Technology Project (201707020003).

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Authors and Affiliations

  1. State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou, 510275, China

    Yan Wang, Qingyun Cai, Jiannan Chen, Zhihong Huang, Wenbi Wu, Meijin Yuan & Kai Yang

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  1. Yan Wang
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  2. Qingyun Cai
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  3. Jiannan Chen
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  4. Zhihong Huang
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  5. Wenbi Wu
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  7. Kai Yang
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Contributions

YW and MY conceived the experiments. YW constructed recombinant viruses and conducted TEM analyses, TCID50 EPDA, BV and ODV purification, IEM analyses, and coimmunoprecipitation assays. QC and JC constructed plasmids. ZH, WW, KY and MY analyzed the results. MY is responsible for the financial support of the project. All authors reviewed the manuscript.

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Correspondence to Meijin Yuan.

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This article does not contain any studies with human or animal subjects performed by any of the authors.

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Wang, Y., Cai, Q., Chen, J. et al. Autographa Californica Multiple Nucleopolyhedrovirus P48 (Ac103) Is Required for the Efficient Formation of Virus-Induced Intranuclear Microvesicles. Virol. Sin. 34, 712–721 (2019). https://doi.org/10.1007/s12250-019-00147-8

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Keywords

  • P48
  • Baculovirus
  • Intranuclear microvesicle formation
  • Protein association
  • Ac93