Oxymatrine Inhibits Bocavirus MVC Replication, Reduces Viral Gene Expression and Decreases Apoptosis Induced by Viral Infection
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Oxymatrine (OMT), as the main active component of Sophoraflavescens, exhibits a variety of pharmacological properties, including anti-oxidative, anti-inflammatory, anti-tumor, and anti-viral activities, and currently is extensively employed to treat viral hepatitis; however, its effects on parvovirus infection have yet to be reported. In the present study, we investigated the effects of OMT on cell viability, virus DNA replication, viral gene expression, cell cycle, and apoptosis in Walter Reed canine cells/3873D infected with minute virus of canines (MVC). OMT, at concentrations below 4 mmol/L(no cellular toxicity), was found to inhibit MVC DNA replication and reduce viral gene expression at both mRNA and protein levels, which was associated with the inhibition of cell cycle S-phase arrest in early-stage of MVC infection. Furthermore, OMT significantly increased cell viability, decreased MVC-infected cell apoptosis, and reduced the expression of activated caspase 3. Our results suggest that OMT has potential application in combating parvovirus infection.
KeywordsOxymatrine (OMT) Minute virus of canines (MVC) Infection Replication Apoptosis
We are thankful to Professor Jianming Qiu (Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, USA) for providing WRD cells and bocavirus MVC, and Huanzhou Xu (a member of Guan’s lab, Wuhan Institute of Virology, CAS, China) for technical help, and Xiangli Hao (School of Foreign Languages, Ningxia Medical University, China) for his assistance in language polishing. This work was funded by the Natural Sciences Foundation of China (31760041) to YS, the West China first-class Disciplines Basic Medical Sciences at Ningxia Medical University (No. NXYLXK2017B07) and Innovative Training Program for College Students (201510752010) to NL.
YS conceived/designed the experiments. YD, NL and JS performed the experiments and analyzed the data. JS, LZ, JG and XH contributed reagents/materials/analysis tools. YS and YD wrote the manuscript. YD and NL prepared the figures and tables. YS checked and finalized the manuscript. All authors read and approved the final manuscript.
Compliance with Ethical Standards
Conflict of interest
The authors declare that they have no conflict of interest.
Animal and Human Rights Statement
This article does not contain any studies with human or animal subjects performed by any of the authors.
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