A Vesicular Stomatitis Virus-Based Vaccine Carrying Zika Virus Capsid Protein Protects Mice from Viral Infection
Zika virus (ZIKV) is a mosquito-borne virus that belongs to the Flavivirus family along with dengue virus (DENV), yellow fever virus, West Nile virus, and Japanese encephalitis virus (Ming et al.2016). ZIKV is a single-stranded positive-sense RNA virus encoding three structural proteins, including nucleocapsid protein C, prM/M, envelope glycoprotein E, and seven non-structural proteins. Since 2015, over 70 countries and territories had reported continuing vector-borne transmission of ZIKV, making it a global public health issue. People infected with ZIKV normally have no or mild symptoms that include fever, rash, muscle pain, red eyes, headache, and conjunctivitis. However, a 2015 survey in Brazil found that the number of microcephaly cases dramatically increased, suggesting that ZIKV infection and newborn deformity were closely related (Heukelbach et al.2016). Additionally, ZIKV infection can cause other severe neurological disorders, such as Guillain–Barré syndrome...
We gratefully thank Dr. Feifei Yin in Hainan Medical University for providing ZIKV PRVABC59 and also like to thank Prof. Yi Shi in Institute of Microbiology, Chinese Academy of Sciences for providing the Zika E monoantibody. This work was supported by the National Natural Science Foundation of China (31470848, 31470880, 31670898, and 31870867), Open Research Fund Program of the State Key Laboratory of Virology of China (2017IOV003) and Jiangsu Provincial Innovative Research Team.
XS, SX, and CD conceived and designed the research. XS, JH, JG, and CW carried out the experiments. SX and CD analyzed the data. SX and CD wrote the paper. All authors have read and approved the final manuscript.
Compliance with Ethical Standards
Conflict of interest
All the authors declare that they have no conflicts of interest.
Animal and Human Rights Statement
All animal experiments were performed in accordance with the guidelines of the Laboratory Animal Ethics Commission of Soochow University.
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