Virologica Sinica

, Volume 34, Issue 1, pp 102–105 | Cite as

Pathogenic Characterization and Full Length Genome Sequence of a Reassortant Infectious Bursal Disease Virus Newly Isolated in Pakistan

  • Altaf Hussain
  • Tiantian Wu
  • Hui Li
  • Linjin Fan
  • Kai Li
  • Li Gao
  • Yongqiang Wang
  • Yulong Gao
  • Changjun Liu
  • Hongyu Cui
  • Qing Pan
  • Yanping Zhang
  • Asim Aslam
  • Khan Muti-Ur-Rehman
  • Muhammad Munir
  • Salman Latif Butt
  • Xiaomei WangEmail author
  • Xiaole QiEmail author

Dear Editor,

Infectious bursal disease (IBD) is one of the most important diseases of the poultry. The IBD virus (IBDV), a non-enveloped virus belonging to the Birnaviridae family with a genome consisting of two segments of double-stranded RNA (segments A and B), targets B lymphocytes of bursa of Fabricious leading to immunosuppression. In Pakistan, poultry farming is the second biggest industry and IBD is the second biggest disease threating the poultry sector. However, there is limited genome information of IBDV available in Pakistan (Shabbir et al.2016).

In March 2017, a suspected IBD outbreak was reported in 28-day-old broiler chicken flock in Punjab, which is the most important chicken-farming area of Pakistan. A total of 30% to 50% morbidity and 13% mortality were observed without previous history of the vaccination. Seventeen (n = 17) bursa samples were collected for virus detection. Using RT-PCR with primers of A628U/A1540L (Supplementary Table S1), a specific fragment of IBDV...



This work was supported by the National Key Research and Development Program of China (Nos. 2016YFE0203200, 2017YFD0500704), the Major Project of National Natural Science Foundation of China (No. 31430087), the Modern Agro-industry Technology Research System (No. CARS-41-G15).

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

Animal and Human Rights Statement

Animal experiments in this study were approved by the Ethics Committees of Harbin Veterinary Research Institute (HVRI), Chinese Academy of Agricultural Sciences (CAAS) (Approval Number: SQ-2017-080).

Supplementary material

12250_2019_82_MOESM1_ESM.pdf (109 kb)
Supplementary material 1 (PDF 109 kb)


  1. Abed M, Soubies S, Courtillon C, Briand FX, Allée C, Amelot M, De Boisseson C, Lucas P, Blanchard Y, Belahouel A (2018) Infectious bursal disease virus in algeria: detection of highly pathogenic reassortant viruses. Infect Genet Evol 60:48–57CrossRefGoogle Scholar
  2. Gao L, Li K, Qi X, Gao H, Gao Y, Qin L, Wang Y, Shen N, Kong X, Wang X (2014) Triplet amino acids located at positions 145/146/147 of the RNA polymerase of very virulent infectious bursal disease virus contribute to viral virulence. J Gen Virol 95:888–897CrossRefGoogle Scholar
  3. He X, Xiong Z, Yang L, Guan D, Yang X, Wei P (2014) Molecular epidemiology studies on partial sequences of both genome segments reveal that reassortant infectious bursal disease viruses were dominantly prevalent in southern china during 2000–2012. Arch Virol 159:3279–3292CrossRefGoogle Scholar
  4. Hernández M, Tomás G, Marandino A, Iraola G, Maya L, Mattion N, Hernández D, Villegas P, Banda A, Panzera Y (2015) Genetic characterization of south american infectious bursal disease virus reveals the existence of a distinct worldwide-spread genetic lineage. Avian Pathol 44:212–221CrossRefGoogle Scholar
  5. Hon CC, Lam TY, Drummond A, Rambaut A, Lee YF, Yip CW, Zeng F, Lam PY, Ng PT, Leung FC (2006) Phylogenetic analysis reveals a correlation between the expansion of very virulent infectious bursal disease virus and reassortment of its genome segment b. J Virol 80:8503–8509CrossRefGoogle Scholar
  6. Jackwood D, Gough R, Sommer S (2005) Nucleotide and amino acid sequence analysis of a birnavirus isolated from penguins. Vet Rec 156:550–552CrossRefGoogle Scholar
  7. Jackwood DJ, Sommer-Wagner SE, Crossley BM, Stoute ST, Woolcock PR, Charlton BR (2011) Identification and pathogenicity of a natural reassortant between a very virulent serotype 1 infectious bursal disease virus (IBDV) and a serotype 2 IBDV. Virology 420:98–105CrossRefGoogle Scholar
  8. Kumar S, Tamura K, Nei M (2004) Mega 3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163CrossRefGoogle Scholar
  9. Le Nouen C, Rivallan G, Toquin D, Darlu P, Morin Y, Beven V, de Boisseson C, Cazaban C, Comte S, Gardin Y (2006) Very virulent infectious bursal disease virus: reduced pathogenicity in a rare natural segment-b-reassorted isolate. J Gen Virol 87:209–216CrossRefGoogle Scholar
  10. Lu Z, Zhang L, Wang N, Chen Y, Gao L, Wang Y, Gao H, Gao Y, Li K, Qi X (2015) Naturally occurring reassortant infectious bursal disease virus in northern china. Virus Res 203:92–95CrossRefGoogle Scholar
  11. Nwagbo IO, Shittu I, Nwosuh CI, Ezeifeka GO, Odibo FJ, Michel LO, Jackwood DJ (2016) Molecular characterization of field infectious bursal disease virus isolates from nigeria. Vet World 9:1420CrossRefGoogle Scholar
  12. Qi X, Gao L, Qin L, Deng X, Wu G, Zhang L, Yu F, Ren X, Gao Y, Gao H (2011) Genomic sequencing and molecular characteristics of a very virulent strain of infectious bursal disease virus isolated in china. Agr Sci Technol 12:1946–1949Google Scholar
  13. Shabbir MZ, Ali M, Abbas M, Chaudhry UN, Munir M (2016) Molecular characterization of infectious bursal disease viruses from pakistan. Arch Virol 161:2001–2006CrossRefGoogle Scholar
  14. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882CrossRefGoogle Scholar
  15. Yuwen Y, Gao Y, Gao H, Qi X, Li T, Liu W, Wang X (2008) Sequence analysis of the VP2 hypervariable region of eight very virulent infectious bursal disease virus isolates from the northeast of china. Avian Dis 52:284–290CrossRefGoogle Scholar

Copyright information

© Wuhan Institute of Virology, CAS 2019

Authors and Affiliations

  1. 1.Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research InstituteThe Chinese Academy of Agricultural SciencesHarbinChina
  2. 2.OIE Reference Laboratory for Infectious Bursal DiseaseHarbinChina
  3. 3.Jiangsu Co-innovation Centre for Prevention and Control of Important Animal Infectious Disease and ZoonosesYangzhouChina
  4. 4.Pathology DepartmentUniversity of Veterinary and Animal SciencesLahorePakistan
  5. 5.Division of Biomedical and Life Sciences, Faculty of Health and MedicineLancaster UniversityLancasterUK
  6. 6.Department of Pathology, College of Veterinary MedicineUniversity of GeorgiaAtlantaUSA

Personalised recommendations