Virologica Sinica

, Volume 34, Issue 1, pp 50–58 | Cite as

Molecular Epidemiology of Echovirus 18 Circulating in Mainland China from 2015 to 2016

  • Xiangpeng Chen
  • Tianjiao Ji
  • Jiayun Guo
  • Wei Wang
  • Wenbo XuEmail author
  • Zhengde XieEmail author
Research Article


Echovirus 18 (E18), a serotype of Enterovirus B (EV-B) species, is an important pathogen in aseptic meningitis. E18 had rarely been detected in mainland China, but became the predominant pathogen associated with viral encephalitis (VE) and meningitis in Hebei province for the first time in 2015. To investigate the molecular epidemiology and genetic characteristics of E18 in mainland China, sixteen E18 strains from patient throat swabs with hand, foot, and mouth disease (HFMD) in six provinces in China collected between 2015 and 2016, and four E18 strains isolated from 18 patient cerebrospinal fluid specimens with VE in Hebei Province in 2015 were obtained and sequenced. Combined with the sequences from the GenBank database, we performed an extensive genetic analysis. Phylogenetic analysis of VP1 gene sequences revealed that all E18 strains from mainland China after 2015 belonged to subgenotype C2. There were no obvious specific differences in phylogenetic and variation analyses of E18 genome sequences between HFMD and VE/meningitis strains. Potential multiple recombination may have occurred in the 5′-untranslated region and in the P2 and P3 nonstructural protein-encoding regions of E18 strains from China. The current E18 strains were potential multiple-recombinant viruses. Overall, these findings supported that E18 caused HFMD, VE, and meningitis, although there were no significant associations between clinical features and viral genomic characteristics.


Genetic characteristics Echovirus 18 (E18) Genome Enterovirus Recombination 



This work was supported by the National Science and Technology Major Project of the Ministry of Science and Technology of China (Grant Nos. 2017ZX10104001-005-010 and 2017ZX10103004-004); Beijing Natural Science Foundation (Grant No. 7184208); Capital Clinical Feature Project of Beijing Technology Program (Grant No. Z151100004015046); Basic and Clinical Research Cooperation Project of Capital Medical University (Grant No. 17JL11); and Research Training Fund of Capital Medical University (Grant No. PYZ2017012). The sponsors had no role in the study design, data analysis, manuscript preparation, or publishing decision.

Author Contributions

XC, ZX, and WX conceived and designed the experiments; XC, TJ, and JG performed the experiments; XC and WW analyzed the data; XC and ZX contributed to the writing of the manuscript. All authors reviewed and approved the final manuscript for submission.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

Animal and Human Rights Statement

The study was approved by the Medical Ethics Committee of Beijing Children’s Hospital, Capital Medical University (Permit No. 2015-8). Informed consents were obtained from legal guardians of all patients prior to the collection of CSF and throat swab samples.

Supplementary material

12250_2018_80_MOESM1_ESM.pdf (204 kb)
Supplementary material 1 (DOCX 204 kb)


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Copyright information

© Wuhan Institute of Virology, CAS 2019

Authors and Affiliations

  1. 1.Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Virology Laboratory, Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical UniversityNational Center for Children’s HealthBeijingChina
  2. 2.WHO WPRO Regional Polio Reference Laboratory and Key Laboratory of Medical Virology and Viral Diseases, National Health and Family Planning Commission of China, National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijingChina

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