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Virologica Sinica

, Volume 33, Issue 6, pp 524–530 | Cite as

Phenotypic Characterization of Porcine IFNγ-Producing Lymphocytes in Porcine Reproductive and Respiratory Syndrome Virus Vaccinated and Challenged Pigs

  • Xiangdong Li
  • Zengyang Pei
  • Yilin Bai
  • Lihua Wang
  • Jishu ShiEmail author
  • Kegong TianEmail author
Research Article
  • 127 Downloads

Abstract

Porcine reproductive and respiratory syndrome (PRRS) continues to be one of the most important swine diseases worldwide. Interferon-γ (IFNγ)-mediated type I cell-mediated immune response plays an important role in protection from, and clearance of, PRRS virus (PRRSV). Several lymphocyte subsets including T-helper, CTLs, Th/memory cells, and γδ T lymphocytes were previously reported to produce IFNγ during PRRSV infection. However, the proportion and phenotypic characterization of these IFNγ-secreting lymphocytes have not been explored. In this study, IFNγ producted by different lymphocyte subsets was assessed by multi-color flow cytometry after vaccination with PRRSV modified live vaccine (PRRSV-MLV) and challenge with homogeneous or heterogeneous PRRSV. The results showed that T-helper cells were the major IFNγ-secreting cell population after PRRSV-MLV vaccination and PRRSV challenge. Additionally, the proportion of IFNγ producing Th/memory cells and γδ T cells increased after PRRSV challenge. This difference was accounted for an enhanced ability to produce IFNγ in Th/memory cells and an enlarged quantity of γδ T cells. The results presented here could contribute to our understanding of the roles of IFNγ in protective immunity against PRRSV infection and may be useful for assessment of cell-mediated immunity in vaccine tests.

Keywords

Porcine reproductive and respiratory syndrome virus (PRRSV) Lymphocyte Interferon-γ (IFNγ) 

Notes

Acknowledgements

This work was supported by Grant from The National Natural Science Foundation of China (NSFC, Grant No. 31490601), National Key Research and Development Program (Grant No. 2016YFD0500703), Major Science and Technology Projects in Henan Province (Grant No. 171100110200), and Luoyang Heluo Talent Plan (Dr. Kegong Tian).

Author Contributions

JS and KT design the study. XL, ZP, YB and LW performed the experiments. XL analyzed the data and drafted the manuscript. XL, JS and KT wrote the manuscript. All authors read and approved the final manuscript.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

Animal and Human Rights Statement

The whole study was approved by the Animal Ethics Committee of National Research Center for Veterinary Medicine in China (Permit No. NVC-2017-018). All institutional and national guidelines for the care and use of laboratory animals were followed.

Supplementary material

12250_2018_73_MOESM1_ESM.pdf (464 kb)
Supplementary material 1 (PDF 464 kb)

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Copyright information

© Wuhan Institute of Virology, CAS 2018

Authors and Affiliations

  1. 1.College of Animal Science and Veterinary MedicineHenan Agricultural UniversityZhengzhouChina
  2. 2.National Research Center for Veterinary MedicineHigh-Tech District, LuoyangChina
  3. 3.Department of Anatomy and Physiology, College of Veterinary MedicineKansas State UniversityManhattanUSA
  4. 4.College of Veterinary MedicineNorthwest A&F UniversityYanglingChina
  5. 5.Department of Veterinary Medicine, College of Animal ScienceZhejiang UniversityHangzhouChina
  6. 6.OIE Porcine Reproductive and Respiratory Syndrome Reference LaboratoryChina Animal Disease Control CenterBeijingChina

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