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Molecular-Genetic Characterization of Human Rotavirus A Strains Circulating in Moscow, Russia (2009–2014)

Virologica Sinica volume 33, pages 304–313 (2018)Cite this article

Abstract

Enteric viruses are the most common cause of acute gastroenteritis (AGE) in young children and a significant public health problem globally. Hospital admissions of children under 5 years of age with diarrhea are primarily associated with group A rotavirus (RVA) infection. In this retrospective study, the population structure of viruses linked to AGE etiology in young children hospitalized with AGE in Moscow was evaluated, and molecular characterization of RVA strains was performed. Fecal specimens were collected from children under 5 years old hospitalized with AGE between 2009 and 2014 in Moscow, Russia. Multiplex real-time reverse transcription PCR was used to detect enteric viruses and for G/[P]-genotyping of isolated RVAs. Sequencing of RVA VP7 and VP4 cDNA fragments was used to validate the data obtained by PCR-genotyping. The main causes for hospitalization of children with AGE were RVA (40.1%), followed by noroviruses (11.4%), while adenoviruses, astroviruses, sapoviruses, enteroviruses, and orthoreoviruses were detected in 4.7%, 1.9%, 1.4%, 1.2%, and 0.2% of samples tested, respectively. Nosocomial infections, predominantly associated with RVAs and noroviruses, were detected in 24.8% of cases and occurred significantly more frequently in younger infants. The predominant RVA genotype was G4P[8], detected in 38.7% of RVA-positive cases, whereas genotypes G1P[8], G9P[8], G3P[8], and G2P[4] were found in 11.8%, 6.6%, 4.2%, and 3.3% of cases, respectively. Together, the presence of circulating RVA strains with rare VP7 and VP4 gene variants (G6 and P[9]) highlights the need to conduct continuous epidemiological monitoring of RVA infection.

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Acknowledgements

The study of the distribution of RVA G/[P]-genotypes was at the expense of the Russian Science Foundation (Grant No. 16-15-10332).

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Authors and Affiliations

  1. Department of Virology, I. Mechnikov Research Institute of Vaccines and Sera, Moscow, 105064, Russia

    Victoria Kiseleva, Evgeny Faizuloev, Anna Marova, Alexey Oksanich, Tatiana Samartseva, Georgy Bakhtoyarov, Nikolay Filatov, Andrey Linok, Yulia Ammour & Vitaly Zverev

  2. Faculty of Preventive Medicine and Health Organization, Russian Medical Academy of Continuous Professional Education, Moscow, 125993, Russia

    Evgeny Faizuloev

  3. M. Vladimirsky Moscow Regional Research Clinical Institute (MONIKI), Moscow, 129110, Russia

    Elena Meskina & Natalia Bochkareva

  4. Faculty of Preventive Medicine, I.M. Sechenov First Moscow State Medical University, Moscow, 119991, Russia

    Nikolay Filatov & Vitaly Zverev

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  1. Victoria Kiseleva
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  2. Evgeny Faizuloev
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  4. Anna Marova
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Contributions

EF and EM designed the experiments. VK, AM, AO, TS, GB, NB and AL carried out the experiments. EF and EM analyzed the data. EF, EM, VK and YA wrote the paper. NF and VZ checked and finalized the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Evgeny Faizuloev.

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Conflict of interest

The authors declared that they have no conflict of interests.

Animal and Human Rights Statement

The study protocol was approved by the Medical Ethics Review Committees of the hospitals in Russia. Written consent was provided by the parents.

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Kiseleva, V., Faizuloev, E., Meskina, E. et al. Molecular-Genetic Characterization of Human Rotavirus A Strains Circulating in Moscow, Russia (2009–2014). Virol. Sin. 33, 304–313 (2018). https://doi.org/10.1007/s12250-018-0043-0

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  • DOI: https://doi.org/10.1007/s12250-018-0043-0

Keywords

  • Acute gastroenteritis (AGE)
  • Rotaviral enteritis
  • Rotavirus vaccine
  • Rotavirus G/[P] genotype
  • Moscow