Molecular-Genetic Characterization of Human Rotavirus A Strains Circulating in Moscow, Russia (2009–2014)
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Enteric viruses are the most common cause of acute gastroenteritis (AGE) in young children and a significant public health problem globally. Hospital admissions of children under 5 years of age with diarrhea are primarily associated with group A rotavirus (RVA) infection. In this retrospective study, the population structure of viruses linked to AGE etiology in young children hospitalized with AGE in Moscow was evaluated, and molecular characterization of RVA strains was performed. Fecal specimens were collected from children under 5 years old hospitalized with AGE between 2009 and 2014 in Moscow, Russia. Multiplex real-time reverse transcription PCR was used to detect enteric viruses and for G/[P]-genotyping of isolated RVAs. Sequencing of RVA VP7 and VP4 cDNA fragments was used to validate the data obtained by PCR-genotyping. The main causes for hospitalization of children with AGE were RVA (40.1%), followed by noroviruses (11.4%), while adenoviruses, astroviruses, sapoviruses, enteroviruses, and orthoreoviruses were detected in 4.7%, 1.9%, 1.4%, 1.2%, and 0.2% of samples tested, respectively. Nosocomial infections, predominantly associated with RVAs and noroviruses, were detected in 24.8% of cases and occurred significantly more frequently in younger infants. The predominant RVA genotype was G4P, detected in 38.7% of RVA-positive cases, whereas genotypes G1P, G9P, G3P, and G2P were found in 11.8%, 6.6%, 4.2%, and 3.3% of cases, respectively. Together, the presence of circulating RVA strains with rare VP7 and VP4 gene variants (G6 and P) highlights the need to conduct continuous epidemiological monitoring of RVA infection.
KeywordsAcute gastroenteritis (AGE) Rotaviral enteritis Rotavirus vaccine Rotavirus G/[P] genotype Moscow
The study of the distribution of RVA G/[P]-genotypes was at the expense of the Russian Science Foundation (Grant No. 16-15-10332).
EF and EM designed the experiments. VK, AM, AO, TS, GB, NB and AL carried out the experiments. EF and EM analyzed the data. EF, EM, VK and YA wrote the paper. NF and VZ checked and finalized the manuscript. All authors read and approved the final manuscript.
Compliance with Ethical Standards
Conflict of interest
The authors declared that they have no conflict of interests.
Animal and Human Rights Statement
The study protocol was approved by the Medical Ethics Review Committees of the hospitals in Russia. Written consent was provided by the parents.
- Bakhtoiarov GN, Kiselev IS, Zverev VV, Faizuloev EB (2014) Evaluation of real-time multiplex PCR effectiveness for group a rotavirus genotyping. Zh Mikrobiol Epidemiol Immunobiol 4:43–49 (in Russian) Google Scholar
- Gleizes O, Desselberger U, Tatochenko V, Rodrigo C, Salman N, Mezner Z, Giaquinto C, Grimprel E (2006) Nosocomial rotavirus infection in European countries: a review of the epidemiology, severity and economic burden of hospital-acquired rotavirus disease. Pediatr Infect Dis J 25:S12–S21CrossRefPubMedGoogle Scholar
- Gurgel RG, Bohland AK, Vieira SC, Oliveira DM, Fontes PB, Barros VF, Ramos MF, Dove W, Nakagomi T, Nakagomi O, Correia JB, Cunliffe N, Cuevas LE (2009) Incidence of rotavirus and all-cause diarrhea in northeast Brazil following the introduction of a national vaccination program. Gastroenterology 137:1970–1975CrossRefPubMedGoogle Scholar
- Hemming M, Rasanen S, Huhti L, Paloniemi M, Salminen M, Vesikari T (2013) Major reduction of rotavirus, but not norovirus, gastroenteritis in children seen in hospital after the introduction of RotaTeq vaccine into the National Immunization Programme in Finland. Eur J Pediatr 172:739–746CrossRefPubMedGoogle Scholar
- Marova AA, Oksanich AS, Kaira AN, Meskina ER, Medvedeva EA, Ivanova OE, Lukashev AN, Kyuregian KK, Kalinkina MA, Egorova OV, Zverev VV, Faizuloev EV (2012) Experience of application of multiplex qPCR for differential diagnostics of intestinal viral infections. Zh Mikrobiol Epidemiol Immunobiol 6:39–45 (in Russian) Google Scholar
- Matthijnssens J, Ciarlet M, Rahman M, Attoui H, Banyai K, Estes MK, Gentsch JR, Iturriza-Gomara M, Kirkwood CD, Martella V, Mertens PP, Nakagomi O, Patton JT, Ruggeri FM, Saif LJ, Santos N, Steyer A, Taniguchi K, Desselberger U, Van Ranst M (2008) Recommendations for the classification of group A rotaviruses using all 11 genomic RNA segments. Arch Virol 153:1621–1629CrossRefPubMedPubMedCentralGoogle Scholar
- Mayindou G, Ngokana B, Sidibe A, Moundele V, Koukouikila-Koussounda F, Christevy Vouvoungui J, Kwedi Nolna S, Velavan TP, Ntoumi F (2016) Molecular epidemiology and surveillance of circulating rotavirus and adenovirus in Congolese children with gastroenteritis. J Med Virol 88:596–605CrossRefPubMedGoogle Scholar
- Omore R, Tate JE, O’Reilly CE, Ayers T, Williamson J, Moke F, Schilling KA, Awuor AO, Jaron P, Ochieng JB, Oundo J, Parashar UD, Parsons MB, Bopp CC, Nasrin D, Farag TH, Kotloff KL, Nataro JP, Panchalingam S, Levine MM, Laserson KF, Nuorti JP, Mintz ED, Breiman RF (2016) Epidemiology, seasonality and factors associated with rotavirus infection among children with moderate-to-severe diarrhea in rural Western Kenya, 2008–2012: the global enteric multicenter study (GEMS). PLoS ONE 11:e0160060CrossRefPubMedPubMedCentralGoogle Scholar
- Parker EPK, Praharaj I, Zekavati A, Lazarus RP, Giri S, Operario DJ, Liu J, Houpt E, Iturriza-Gomara M, Kampmann B, John J, Kang G, Grassly NC (2018) Influence of the intestinal microbiota on the immunogenicity of oral rotavirus vaccine given to infants in south India. Vaccine 36:264–272CrossRefPubMedPubMedCentralGoogle Scholar
- Payne DC, Vinje J, Szilagyi PG, Edwards KM, Staat MA, Weinberg GA, Hall CB, Chappell J, Bernstein DI, Curns AT, Wikswo M, Shirley SH, Hall AJ, Lopman B, Parashar UD (2013) Norovirus and medically attended gastroenteritis in US children. N Engl J Med 368:1121–1130CrossRefPubMedPubMedCentralGoogle Scholar
- Podkolzin AT, Fenske EB, Abramycheva NY, Shipulin GA, Sagalova OI, Mazepa VN, Ivanova GN, Semena AV, Tagirova ZG, Alekseeva MN, Molochny VP, Parashar UD, Vinje J, Maleev VV, Glass RI, Pokrovsky VI (2009) Hospital-based surveillance of rotavirus and other viral agents of diarrhea in children and adults in Russia, 2005–2007. J Infect Dis 200(Suppl 1):S228–S233CrossRefPubMedGoogle Scholar
- Shim JO, Chang JY, Shin S, Moon JS, Ko JS (2016) Changing distribution of age, clinical severity, and genotypes of rotavirus gastroenteritis in hospitalized children after the introduction of vaccination: a single center study in Seoul between 2011 and 2014. BMC Infect Dis 16:287CrossRefPubMedPubMedCentralGoogle Scholar
- Tam CC, O’Brien SJ, Tompkins DS, Bolton FJ, Berry L, Dodds J, Choudhury D, Halstead F, Iturriza-Gomara M, Mather K, Rait G, Ridge A, Rodrigues LC, Wain J, Wood B, Gray JJ (2012) Changes in causes of acute gastroenteritis in the United Kingdom over 15 years: microbiologic findings from 2 prospective, population-based studies of infectious intestinal disease. Clin Infect Dis 54:1275–1286CrossRefPubMedGoogle Scholar
- Zhirakovskaia EV, Aksanova R, Gorbunova MG, Tikunov A, Kuril’shchikov AM, Sokolov SN, Netesov SV, Tikunova NV (2012) Genetic diversity of group A rotavirus isolates found in Western Siberia in 2007–2011. Mol Gen Mikrobiol Virusol 4:33–41Google Scholar