Exhaustive Exercise Does Not Affect Humoral Immunity and Protection after Rabies Vaccination in a Mouse Model
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Rabies is one of the most dangerous and widespread zoonosis and is characterized by severe neurological signs and a high case-mortality rate of nearly 100%. Vaccination is the most effective way to prevent rabies in humans and animals. In this study, the relationship between exhaustive exercise and the humoral immune response after immunization with inactivated rabies vaccine was investigated in a mouse model with one-time exhaustive exercise. It was found that compared with the mice with no exercise after vaccination, no significant differences were found in those with exhaustive exercise after vaccination on body-weight changes, virus-neutralizing antibody (VNA) titers, antibody subtypes and survivor ratio after lethal rabies virus (RABV) challenge. This study indicated that exhaustive exercise does not reduce the effects of the rabies inactivated vaccine.
KeywordsRabies Vaccination Exhaustive exercise Virus-neutralizing antibody Protection
This work was partially supported by the National Natural Science Foundation of China (31702248, 31402176, 31372419 and 31522057), the National Science Foundation for Post-doctoral Scientists of China (Grant No. 20163M590701), the National Program on Key Research Project of China (2016YFD0500400), the Fundamental Research Funds for the Central Universities (2662016QD036, to MZ) and the Ministry of Agriculture of China (special fund for Agro-scientific research in the Public Interest, 201303042).
ZFF, LZ, MZ, DYT conceived and designed the experiments. LX, MRL, YJ Z, JCR, JP, JLS performed the experiments. DYT, LZ, MZ analyzed the data. LX, DYT, MZ wrote the manuscript and prepared the Figures. ZFF, LZ, MZ, DYT checked and finalized the manuscript. All authors read and approved the final manuscript.
Compliance with Ethics Standards
Conflict of interest
The authors declare that they have no conflict of interest.
Animal and Human Rights Statement
Animal experiments in this study were approved by the Scientific Ethics Committee of Huazhong Agricultural University (permit number HZAUMO-2016-047).
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