Virologica Sinica

, Volume 33, Issue 1, pp 111–115 | Cite as

Development of Multi-analyte Suspension Assay for Simultaneously Efficient Detection of Avian Influenza Virus A Subtypes

  • Yi Li
  • Longquan Ni
  • Jianjun Chen
  • Juan Yang
  • Fei Deng
  • Hualin WangEmail author

Dear Editor,

Aquatic birds are thought to be the main reservoir for avian influenza viruses (AIVs) (Olsen et al. 2006). Pandemic emergences caused by these viruses threaten the international poultry industry giving rise to enormous economic losses. In addition, more and more reported cases of bird-to-human transmission alert us the importance of AIVs surveillance in natural foci for identifying, monitoring, and predicting the emergence of pathogens that have a potential threat (Poovorawan et al. 2013; Subbarao et al. 1998). Influenza A viruses are enveloped viruses with eight distinct negative-strand RNA segments which belong to the Orthomyxoviridaefamily. The RNA segments 4 and 6 encode for hemagglutinin (HA) and neuraminidase (NA) glycoproteins respectively. HAs are viral surface proteins that bind to sialic acid residues at the host cell surface and ensure further virus entry. NAs are considered to be responsible for release of progeny virions. Based on the antigenic properties of...



This work was supported by grants from the National Natural Science Foundation of China (No. 81501430) and the Basic Work Program of the Ministry of Science and Technology of China (2013FY113500).

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Animal and Human Rights Statement

This article does not contain any studies with human or animal subjects performed by any of the authors.

Supplementary material

12250_2018_18_MOESM1_ESM.pdf (120 kb)
Supplementary material 1 (PDF 129 kb)
12250_2018_18_MOESM2_ESM.xlsx (582 kb)
Supplementary Table S1 Accession numbers of 27,549 Asian AIVs subtypes sequences downloaded from NCBI and GISAID database (XLSX 582 kb)


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Copyright information

© Wuhan Institute of Virology, CAS and Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  1. 1.State Key Laboratory of Virology, Wuhan Institute of VirologyChinese Academy of SciencesWuhanChina
  2. 2.CAS Key Laboratory of Special Pathogens and BiosafetyWuhan Institute of Virology, Chinese Academy of SciencesWuhanChina

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