Virologica Sinica

, Volume 32, Issue 6, pp 537–540 | Cite as

An improved method for identifying SUMOylation sites of viral proteins

  • Yan Liu
  • Zhongyuan Tan
  • Bo Shu
  • Yuan Zhang
  • Caishang Zheng
  • Xianliang Ke
  • Xi Chen
  • Hanzhong Wang
  • Zhenhua Zheng

In this study, we improved the most commonly used methods for MS detection of SUMOylated sites and used an E. coli recombination SUMOylation system with SUMO-1 (T95R). This system provides fast enrichment of SUMOylated viral protein in less than 2 days, and shows advantage over the method of collecting modified protein from cells in convenience and sensitivity. Furthermore, this method provides an option for rapid and accurate identification of the potential viral protein SUMOylation sites.


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Supplementary material

12250_2017_4018_MOESM1_ESM.pdf (3.6 mb)
An improved method for identifying SUMOylation sites of viral proteins


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Copyright information

© Wuhan Institute of Virology, CAS and Springer Nature Singapore Pte Ltd. 2017

Authors and Affiliations

  1. 1.CAS Key Laboratory of Special Pathogens and Biosafety, Center for Emerging Infectious Diseases, Wuhan Institute of VirologyChinese Academy of SciencesWuhanChina
  2. 2.Wuhan Institutes of BiotechnologyWuhanChina

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