Virologica Sinica

, Volume 32, Issue 3, pp 199–206

Development of a reverse transcription quantitative polymerase chain reaction-based assay for broad coverage detection of African and Asian Zika virus lineages

Research Article


The Zika virus (ZIKV) is an arbovirus that has spread rapidly worldwide within recent times. There is accumulating evidence that associates ZIKV infections with Guillain-Barré Syndrome (GBS) and microcephaly in humans. The ZIKV is genetically diverse and can be separated into Asian and African lineages. A rapid, sensitive, and specific assay is needed for the detection of ZIKV across various pandemic regions. So far, the available primers and probes do not cover the genetic diversity and geographic distribution of all ZIKV strains. To this end, we have developed a one-step quantitative reverse transcription polymerase chain reaction (qRT-PCR) assay based on conserved sequences in the ZIKV envelope (E) gene. The detection limit of the assay was determined to be five RNA transcript copies and 2.94 × 10–3 50% tissue culture infectious doses (TCID50) of live ZIKV per reaction. The assay was highly specific and able to detect five different ZIKV strains covering the Asian and African lineages without nonspecific amplification, when tested against other flaviviruses. The assay was also successful in testing for ZIKV in clinical samples. Our assay represents an improvement over the current methods available for the detection ZIKV and would be valuable as a diagnostic tool in various pandemic regions.


Flavivirus Zika virus(ZIKV) molecular diagnostics qRT-PCR 


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Copyright information

© Wuhan Institute of Virology, CAS and Springer Science+Business Media Singapore 2017

Authors and Affiliations

  1. 1.Shenzhen Key Laboratory of Pathogen and Immunity, State Key Discipline of Infectious DiseaseShenzhen Third People’s HospitalShenzhenChina
  2. 2.CAS Key Laboratory of Pathogenic Microbiology and Immunology, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Disease, Institute of Microbiology, Center for Influenza Research and Early-warning (CASCIRE)Chinese Academy of SciencesBeijingChina
  3. 3.China-Japan Union Hospital of Jilin UniversityChangchunChina
  4. 4.Key Laboratory for Medical Virology, NHFPC, National Institute for Viral Disease Control and PreventionChina CDCBeijingChina
  5. 5.Office of Director-GeneralChinese Center for Disease Control and PreventionBeijingChina
  6. 6.Savaid Medical SchoolUniversity of Chinese Academy of SciencesBeijingChina

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