Virologica Sinica

, Volume 31, Issue 5, pp 363–379

Three-dimensional cell culture models for investigating human viruses


DOI: 10.1007/s12250-016-3889-z

Cite this article as:
He, B., Chen, G. & Zeng, Y. Virol. Sin. (2016) 31: 363. doi:10.1007/s12250-016-3889-z


Three-dimensional (3D) culture models are physiologically relevant, as they provide reproducible results, experimental flexibility and can be adapted for high-throughput experiments. Moreover, these models bridge the gap between traditional two-dimensional (2D) monolayer cultures and animal models. 3D culture systems have significantly advanced basic cell science and tissue engineering, especially in the fields of cell biology and physiology, stem cell research, regenerative medicine, cancer research, drug discovery, and gene and protein expression studies. In addition, 3D models can provide unique insight into bacteriology, virology, parasitology and host-pathogen interactions. This review summarizes and analyzes recent progress in human virological research with 3D cell culture models. We discuss viral growth, replication, proliferation, infection, virus-host interactions and antiviral drugs in 3D culture models.


three-dimensional (3D) cell culture models scaffolds human viruses 

Copyright information

© Wuhan Institute of Virology, CAS and Springer Science+Business Media Singapore 2016

Authors and Affiliations

  1. 1.State Key Laboratory of Infection Disease Prevention and Control, National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijingChina

Personalised recommendations