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Ficolin-2 binds to HIV-1 gp120 and blocks viral infection

  • Research Article
  • Published:
Virologica Sinica

Abstract

Ficolin-2 is a lectin complement pathway activator present in normal human plasma and usually associated with infectious diseases, but little is known about the role of ficolin-2 in human immunodeficiency virus (HIV) infection. Here, we describe our novel findings that serum ficolin-2 concentrations of 103 HIV-1 patients were much higher compared to those of 57 healthy donors. In vitro analysis showed that HIV-1 infection could enhance ficolin-2 expression. We further demonstrated that recombinant ficolin-2 protein could bind with HIV-1 envelope glycoprotein gp120, and subsequently induce complement dependent cytotoxicity. Moreover, ficolin-2 could block the entry of HIV-1 into target cells (TZM-b1 and MT-2 cells) and infection in a ficolin-2 dosedependent manner. To our knowledge, this is the first report about the protective role of ficolin-2 against HIV-1 infection and our study suggests that ficolin-2 is an important human innate immune molecule against HIV.

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Authors and Affiliations

  1. The State Key Laboratory of Virology, Department of Immunology and Hubei Province Key Laboratory of Allergy and Immune-related Diseases, Wuhan University School of Medicine, Wuhan, 430071, China

    Fengling Luo, Tielong Chen, Jun Liu, Yinnan Zhao & Xiaolian Zhang

  2. Zhongnan Hospital of Wuhan University, Wuhan, 430071, China

    Tielong Chen

  3. Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China

    Xihui Shen & Rongge Yang

Authors
  1. Fengling Luo
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  2. Tielong Chen
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  3. Jun Liu
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  4. Xihui Shen
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  5. Yinnan Zhao
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  6. Rongge Yang
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  7. Xiaolian Zhang
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Corresponding authors

Correspondence to Rongge Yang or Xiaolian Zhang.

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ORCID: 0000-0002-6333-7941

ORCID: 0000-0002-8283-9381

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Luo, F., Chen, T., Liu, J. et al. Ficolin-2 binds to HIV-1 gp120 and blocks viral infection. Virol. Sin. 31, 406–414 (2016). https://doi.org/10.1007/s12250-016-3808-3

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  • DOI: https://doi.org/10.1007/s12250-016-3808-3

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