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Virologica Sinica

, Volume 31, Issue 1, pp 69–77 | Cite as

Detection and characterization of diverse alpha- and betacoronaviruses from bats in China

  • Lin Xu
  • Fuqiang Zhang
  • Weihong Yang
  • Tinglei Jiang
  • Guanjun Lu
  • Biao He
  • Xingyu Li
  • Tingsong Hu
  • Gang Chen
  • Yun Feng
  • Yuzhen Zhang
  • Quanshui Fan
  • Jiang Feng
  • Hailin Zhang
  • Changchun Tu
Research Article

Abstract

Bats have been implicated as important reservoir hosts of alpha- and betacoronaviruses. In this study, diverse coronaviruses (CoVs) were detected in 50 of 951 (positive rate 5.3%) intestinal specimens of eight bat species collected in four provinces and the Tibet Autonomous Region of China by pan-coronavirus RT-PCR screening. Based on 400-nt RNA-dependent RNA polymerase (RdRP) sequence analysis, eight belonged to genus Alphacoronavirus and 42 to Betacoronavirus. Among the 50 positive specimens, thirteen gave rise to CoV full-length RdRP gene amplification for further sequence comparison, of which three divergent sequences (two from a unreported province) were subjected to full genome sequencing. Two complete genomes of betacoronaviruses (JTMC15 and JPDB144) and one nearly-complete genome of alphacoronavirus (JTAC2) were sequenced and their genomic organization predicted. The present study has identified additional numbers of genetically diverse bat-borne coronaviruses with a wide distribution in China. Two new species of bat CoV, identified through sequence comparison and phylogenetic analysis, are proposed.

Keywords

bats Alphacoronavirus Betacoronavirus diversity 

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Copyright information

© Wuhan Institute of Virology, CAS and Springer Science+Business Media Singapore 2016

Authors and Affiliations

  • Lin Xu
    • 1
  • Fuqiang Zhang
    • 2
  • Weihong Yang
    • 3
  • Tinglei Jiang
    • 4
  • Guanjun Lu
    • 4
  • Biao He
    • 1
  • Xingyu Li
    • 1
  • Tingsong Hu
    • 2
  • Gang Chen
    • 2
  • Yun Feng
    • 3
  • Yuzhen Zhang
    • 3
  • Quanshui Fan
    • 2
  • Jiang Feng
    • 4
  • Hailin Zhang
    • 3
  • Changchun Tu
    • 1
    • 5
  1. 1.Military Veterinary InstituteAcademy of Military Medical SciencesChangchunChina
  2. 2.Center for Disease Control and Prevention of Chengdu Military RegionKunmingChina
  3. 3.Yunnan Institute of Endemic Diseases Control and PreventionDaliChina
  4. 4.School of Urban and Environmental SciencesNortheast Normal UniversityChangchunChina
  5. 5.Jiangsu Co-innovation Center for Prevention and Control of ImportantAnimal Infectious Diseases and ZoonosesYangzhouChina

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