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Virologica Sinica

, Volume 30, Issue 3, pp 190–199 | Cite as

A respiratory syncytial virus persistent-infected cell line system reveals the involvement of SOCS1 in the innate antiviral response

  • Junwen Zheng
  • Pu Yang
  • Yan Tang
  • Dongchi ZhaoEmail author
Research Article

Abstract

HEp-2 cells persistently infected with respiratory syncytial virus (RSV) are a heterogeneous mixture of viral antigen-positive and -negative variants; however, the mechanism through which viral replication becomes latent remains unclear. In this study, we investigated the potential mechanism by which RSV escapes from innate immune surveillance. Persistent-infected RSV HEp-2 cells were isolated and cell clones were passaged. The RSV-persistent cells produced viruses at a lower titer, resisted wild-type RSV re-infection, and secreted high levels of interferon-ß (IFN-ß), macrophage inflammatory protein-1α (Mip-1α), interleukin-8 (IL-8), and Rantes. Toll-like receptor 3 (TLR3), retinoic acid inducible gene-I (RIG-I), and suppressor of cytokine signaling 1 (SOCS1) levels were upregulated in these cells. The silencing of TLR3 mRNA decreased the expression of SOCS1 protein and the secretion of cytokines. RSV-persistent cells are in an inflammatory state; upregulation of SOCS1 is related to the TLR3 signaling pathway, which could be associated with the mechanism of viral persistence.

Keywords

respiratory syncytial virus (RSV) suppressor of cytokine signaling 1 (SOCS1) interferon toll-like receptor 3 (TLR3) RSV-persistent cell line 

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Copyright information

© Wuhan Institute of Virology, CAS and Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  1. 1.Department of PediatricsZhongnan Hospital of Wuhan UniversityWuhanChina
  2. 2.Department of Anatomy, School of MedicineWuhan UniversityWuhanChina

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