Virologica Sinica

, Volume 29, Issue 6, pp 372–380 | Cite as

Nucleoprotein-based indirect enzyme-linked immunosorbent assay (indirect ELISA) for detecting antibodies specific to Ebola virus and Marbug virus

  • Yi Huang
  • Youjie Zhu
  • Mengshi Yang
  • Zhenqing Zhang
  • Donglin Song
  • Zhiming Yuan
Research Article

Abstract

Full-length nucleoproteins from Ebola and Marburg viruses were expressed as His-tagged recombinant proteins in Escherichia coli and nucleoprotein-based enzyme-linked immunosorbent assays (ELISAs) were established for the detection of antibodies specific to Ebola and Marburg viruses. The ELISAs were evaluated by testing antisera collected from rabbit immunized with Ebola and Marburg virus nucleoproteins. Although little cross-reactivity of antibodies was observed in anti-Ebola virus nucleoprotein rabbit antisera, the highest reactions to immunoglobulin G (IgG) were uniformly detected against the nucleoprotein antigens of homologous viruses. We further evaluated the ELISA’s ability to detect antibodies to Ebola and Marburg viruses using human sera samples collected from individuals passing through the Guangdong port of entry. With a threshold set at the mean plus three standard deviations of average optical densities of sera tested, the ELISA systems using these two recombinant nucleoproteins have good sensitivity and specificity. These results demonstrate the usefulness of ELISA for diagnostics as well as ecological and serosurvey studies of Ebola and Marburg virus infection.

Keywords

Ebola virus Marburg virus recombinant nucleoproteins indirect ELISA 

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Copyright information

© Wuhan Institute of Virology, CAS and Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Yi Huang
    • 1
  • Youjie Zhu
    • 1
    • 2
  • Mengshi Yang
    • 1
  • Zhenqing Zhang
    • 1
  • Donglin Song
    • 1
  • Zhiming Yuan
    • 1
  1. 1.Wuhan Institute of VirologyChinese Academy of SciencesWuhanChina
  2. 2.University of the Chinese Academy of SciencesBeijingChina

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