Virologica Sinica

, Volume 29, Issue 5, pp 274–283 | Cite as

Role of the virion host shutoff protein in neurovirulence of monkey B virus (Macacine herpesvirus 1)

  • Darla Black
  • Jerry Ritchey
  • Mark Payton
  • Richard Eberle
Research Article


Monkey B virus (Macacine herpesvirus 1; BV) is noted for its extreme neurovirulence in humans. Since the vhs protein encoded by the UL41 gene has been shown to be a neurovirulence factor in the related human herpes simplex viruses, the role of the UL41 gene in BV neurovirulence was investigated. BV mutants were constructed that lacked the entire UL41 ORF (Δ41) or had the RNase active site mutated (Δ41A). Neither mutant shut off host protein synthesis, degraded β-actin mRNA, or prevented an IFN-β response, indicating that the vhs protein and its RNase activity are both necessary for these activities. Replication of both mutants in primary mouse cells was impaired and they exhibited a prolonged disease course in mice. Whereas Δ41 infected mice were euthanized for symptoms related to central nervous system (CNS) infection, Δ41A infected mice were euthanized primarily for symptoms of autonomic nervous system dysfunction. While neuroinvasiveness was not affected, lesions in the CNS were more limited in size, anatomical distribution, and severity than for wild-type virus. These results indicate that the vhs protein affects the general replicative efficiency of BV in vivo rather than being a specific neurovirulence factor critical for invasion of or preferential replication in the CNS.


herpesvirus monkey B virus UL41 virion host shutoff neurovirulence 


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Copyright information

© Wuhan Institute of Virology, CAS and Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Darla Black
    • 1
  • Jerry Ritchey
    • 1
  • Mark Payton
    • 2
  • Richard Eberle
    • 1
  1. 1.Department of Veterinary Pathobiology, Center for Veterinary Health SciencesOklahoma State UniversityOklahomaUSA
  2. 2.Department of StatisticsOklahoma State UniversityStillwaterUSA

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