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Virologica Sinica

, Volume 29, Issue 4, pp 199–210 | Cite as

Labeling of influenza viruses with synthetic fluorescent and biotin-labeled lipids

  • Natalia A. IlyushinaEmail author
  • Evgeny S. Chernyy
  • Elena Y. Korchagina
  • Aleksandra S. Gambaryan
  • Stephen M. Henry
  • Nicolai V. BovinEmail author
Research Article

Abstract

Direct labeling of virus particles is a powerful tool for the visualization of virus-cell interaction events. However, this technique involves the chemical modification of viral proteins that affects viral biological properties. Here we describe an alternative approach of influenza virus labeling that utilizes Function-Spacer-Lipid (FSL) constructs that can be gently inserted into the virus membrane. We assessed whether labeling with fluorescent (fluo-Ad-DOPE) or biotin-labeled (biot-CMG2-DOPE) probes has any deleterious effect on influenza virus hemagglutinin (HA) receptor specificity, neuraminidase (NA) activity, or replicative ability in vitro. Our data clearly show that neither construct significantly affected influenza virus infectivity or viral affinity to sialyl receptors. Neither construct influenced the NA activities of the influenza viruses tested, except the A/Puerto Rico/8/34 (H1N1) strain. Our data indicate that lipid labeling provides a powerful tool to analyze influenza virus infection in vitro.

Keywords

bioimaging Function-Spacer-Lipid (FSL) constructs labeling of influenza viruses synthetic lipids 

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Copyright information

© Wuhan Institute of Virology, CAS and Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Natalia A. Ilyushina
    • 1
    Email author
  • Evgeny S. Chernyy
    • 2
  • Elena Y. Korchagina
    • 2
  • Aleksandra S. Gambaryan
    • 3
  • Stephen M. Henry
    • 4
  • Nicolai V. Bovin
    • 2
    Email author
  1. 1.Food and Drug AdministrationBethesdaUSA
  2. 2.Shemyakin Institute of Bioorganic ChemistryRussian Academy of SciencesMoscowRussia
  3. 3.Chumakov Institute of Poliomyelitis and Viral EncephalitidesRussian Academy of Medical SciencesMoscow RegionRussia
  4. 4.Biotechnology Research InstituteAUT UniversityAucklandNew Zealand

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