Advertisement

Virologica Sinica

, Volume 29, Issue 4, pp 242–249 | Cite as

Association of swine influenza H1N1 pandemic virus (SIV-H1N1p) with porcine respiratory disease complex in sows from commercial pig farms in Colombia

  • Luisa Fernanda Mancipe JiménezEmail author
  • Gloria Ramírez Nieto
  • Victor Vera Alfonso
  • Jairo Jaime CorreaEmail author
Research Article

Abstract

Porcine respiratory disease complex (PRDC) is a serious health problem that mainly affects growing and finishing pigs. PRDC is caused by a combination of viral and bacterial agents, such as porcine reproductive and respiratory syndrome virus (PRRSV), swine influenza virus (SIV), Mycoplasma hyopneumoniae (Myh), Actinobacillus pleuropneumoniae (APP), Pasteurella multocida and Porcine circovirus 2 (PCV2). To characterize the specific role of swine influenza virus in PRDC presentation in Colombia, 11 farms from three major production regions in Colombia were examined in this study. Nasal swabs, bronchial lavage and lung tissue samples were obtained from animals displaying symptoms compatible with SIV. Isolation of SIV was performed in 9-day embryonated chicken eggs or Madin-Darby Canine Kidney (MDCK) cells. Positive isolates, identified via the hemagglutination inhibition test, were further analyzed using PCR. Overall, 7 of the 11 farms were positive for SIV. Notably, sequencing of the gene encoding the hemagglutinin (HA) protein led to grouping of strains into circulating viruses identified during the human outbreak of 2009, classified as pandemic H1N1-2009. Serum samples from 198 gilts and multiparous sows between 2008 and 2009 were obtained to determine antibody presence of APP, Myh, PCV2 and PRRSV in both SIV-H1N1p-negative and -positive farms, but higher levels were recorded for SIV-H1N1p-positive farms. Odds ratio (OR) and P values revealed statistically significant differences (p<0.05) in PRDC presentation in gilts and multiparous sows of farms positive for SIV-H1N1p. Our findings indicate that positive farms have increased risk of PRDC presentation, in particular, PCV2, APP and Myh.

Keywords

SIV-H1N1 swine influenza pandemic porcine respiratory disease complex Colombia 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bossé J T, Janson H, Sheehan B J, Beddek A J, Rycroft A N, Kroll J S, Langford P R, 2002. Actinobacillus pleuropneumoniae: pathobiology and pathogenesis of infection. Microbes Infect, 4: 225–235.PubMedCrossRefGoogle Scholar
  2. Chiou M T, Lin C N, Chuang M S, Chiang H C, Tsai C P. 2004. Etiological and epidemiological survey of PRDC associated pathogens in Taiwan. In: Proceedings of the 18th International Pig Veterinary Society Congress, Hamburg, p191.Google Scholar
  3. Choi Y, Goyal S, Kang S, Farnham M, Joo H. 2002. Detection and subtyping of swine influenza H1N1, H1N2 and H3N2 viruses in clinical samples using two multiplex RT-PCR assays. J Virol Methods, 102: 53–59.PubMedCrossRefGoogle Scholar
  4. Choi Y K, Goyal S M, Joo H S. 2003. Retrospective analysis of etiologic agents associated with respiratory diseases in pigs. Can Vet J, 44: 735–737.PubMedCentralPubMedGoogle Scholar
  5. Dee S. 1996. The porcine respiratory disease complex: are subpopulations important? J Swine Health Prod, 4: 147–149.Google Scholar
  6. Dorr P M, Baker R B, Almond G W, Wayne S R, Gebreyes W A. 2007. Epidemio-logic assessment of porcine circovirus type 2 coinfection with other pathogens in swine. J Am Vet Med Assoc, 230: 244–250.PubMedCrossRefGoogle Scholar
  7. Easterday B C, Reeth K, Straw B E. 1999. Swine influenza. In: Diseases of Swine. 8th ed. Straw B E, Zimmerman J J, D’ Allaire S, Taylor D J, eds. Iowa: Iowa State University Press. p1209.Google Scholar
  8. Fachinger V, Bischoff R, Jedidia S B, Saalmuller A, Elbers K. 2008. The effect of vaccination against porcine circovirus type 2 in pigs suffering from porcine respiratory disease complex. Vaccine, 26: 1488–1499.PubMedCrossRefGoogle Scholar
  9. Fouchier R, Munster V, Wallensten A, Bestebroer T, Herfst S, Smith D, Rimmelzwaan G, Olsen B, Osterhaus A. 2005. Characterization of a Novel Influenza A Virus Hemagglutinin Subtype (H16) Obtained from Black-Headed Gull. J Virol, 79: 2814–2822.PubMedCentralPubMedCrossRefGoogle Scholar
  10. Gramer M. 2008. An update on swine influenza ecology and diagnostics. American Association of Swine Veterinarians. Vet Rec, 162: 267–271.Google Scholar
  11. Gucht S V, Labarque G, Van Reeth K. 2004. The combination of PRRSV virus and bacterial endotoxin as a model for multifactorial respiratory disease in pigs. Vet Immunol Immunopathol, 102: 165–178.PubMedCrossRefGoogle Scholar
  12. Gutiérrez C, Rodríguez O, Alvarez D, Puente V, García F, Martín J, Rodríguez E. 2000. Simultaneous serological evidence of Actinobacillus pleuropneumoniae, PRRSV, Aujeszky’s disease and influenza viruses in Spanish finishing pigs. Res Vet Sci, 68: 9–13.CrossRefGoogle Scholar
  13. Hansen M S, Pors S E, Jensen H E, Bille-Hansen V, Bisgaard M, Flachs E M, Nielsen E M. 2010. An Investigation of the Pathology and Pathogens Associated with Porcine Respiratory Disease Complex in Denmark. J Comp Path Vol, 143: 120–131.CrossRefGoogle Scholar
  14. Harms P A, Halbur P G, Sorden S D. 2002. Three cases of porcine respiratory disease complex associated with porcine circovirus type 2 infection. J Swine Health Prod, 10: 27–30.Google Scholar
  15. Kim J, Chung H K, Chae C. 2003. Association of porcine circovirus 2 with porcine respiratory disease complex. Vet J, 166: 251–256.PubMedCrossRefGoogle Scholar
  16. Kitikoon P, Vincent A, Jones K, Nilubol D, Yu S, Janke B, Thacker B, Thacker E. 2009. Vaccine efficacy and immune response to swine influenza virus challenge in pigs infected with porcine reproductive and respiratory syndrome virus at the time of SIV vaccination. Vet Microbiol, 139: 235–244.PubMedCrossRefGoogle Scholar
  17. Kuntz-Simon G, Madec F. 2009, Genetic and Antigenic Evolution of Swine Influenza Viruses in Europe and Evaluation of Their Zoonotic Potential. Zoonoses Public Hlth, 56: 310–325.CrossRefGoogle Scholar
  18. Opriessnig T, Meng X J, Halbur P G. 2007. Porcine circovirus type 2 associated disease: update on current terminology, clinical manifestations, pathogenesis, diagnosis, and intervention strategies. J Vet Diagn Invest, 19: 591–615.PubMedCrossRefGoogle Scholar
  19. Opriessnig T, Halbur P G. 2012. Concurrent infections are important for expression of porcine circovirus associated disease. Virus Res, 164: 20–32.PubMedCrossRefGoogle Scholar
  20. Pallarés F J, Halbur P G, Opriessnig T, Sorden S D, Villar D, Janke B H, Yaeger M J, Larson D J, Schwartz K J, Yoon K J, Hoffman L J. 2002. Porcine circovirus type 2 (PCV-2) coinfections in US field cases of postweaning multisystemic wasting syndrome (PMWS). J. Vet. Diagn. Invest, 14: 515–519.PubMedCrossRefGoogle Scholar
  21. Palzer A, Ritzmann M, Wolf G, Heinritzi K. 2008. Associations between pathogens in healthy pigs and pigs with pneumonia. Vet Rec, 1 62: 267–271.PubMedCrossRefGoogle Scholar
  22. Pereda A, Cappuccio J, Quiroga M A, Baumeister E, Insarralde L, Ibar M. 2010. Pandemic (H1N1) 2009 outbreak on pig farm, Argentina. Emerg Infect Dis, 16: 304–307.PubMedCrossRefGoogle Scholar
  23. Pol J M, van Leengoed L A, Stockhofe N, Kok G, Wensvoort G, 1997. Dual infections of PRRSVV/influenza or PRRSVV/Actinobacillus pleuropneumoniae in the respiratory tract. Vet Microbiol, 55: 259–264.PubMedCrossRefGoogle Scholar
  24. Prieto C, Suarez P, Simarro I, Garcia C, Fernandez A, Castro J M, 1997. Transplacental infection following exposure of gilts to porcine reproductive and respiratory syndrome virus at the onset of gestation. Vet Microbiol, 57: 301–311.PubMedCrossRefGoogle Scholar
  25. Ramirez-Nieto G, Rojas C, Alfonso V, Correa J, Galvis J. 2012. First isolation and identification of H1N1 swine influenza viruses in Colombian pig farms. Health, 4: 983–990.CrossRefGoogle Scholar
  26. Reeth V K, Vleeschauwer A, Kyriakis C, Pensaert M. 2006. Influenza in Birds, Pigs and Humans: Old Theories versus Current Viewpoints. Proceedings of the 19th IPVS congress, Denmark, Volume 1.Google Scholar
  27. Saitou N, Nei M. 1987. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol Biol Evol, 4: 406–425.PubMedGoogle Scholar
  28. Sreta D, Tantawet S, Ayudhya S, Thontiravong A, Wongphatcharachai M, Lapkuntod J. 2010. Pandemic (H1N1) 2009 Virus on Commercial Swine Farm, Thailand. Emerg Infect Dis, 16:1587–1590.PubMedCentralPubMedCrossRefGoogle Scholar
  29. Taylor D J. 1999. Actinobacillus pleuropneumoniae. In: Diseases of Swine, Shaw B E, D’Allaire S, Mengeling W L, Taylor D J, (Eds.), Oxford: Blackwell Science, pp343–354.Google Scholar
  30. Thacker E L, Halbur P G, Ross R F, Thanawongnuwech R, Thacker B J. 1999. Mycoplasma hyopneumoniae potentiation of porcine reproductive and respiratory syndrome virus-induced pneumonia. J Clin Microbiol, 37: 620–627.PubMedCentralPubMedGoogle Scholar
  31. Thacker E L. 2001. Immunology of the porcine respiratory disease complex. Immunology, 17: 551–565.Google Scholar
  32. Thacker E L. 2006. Mycoplasmal diseases. In: Diseases of Swine, 9th Ed, Straw B E, Zimmerman J J, D’Allaire S, Taylor D J, Eds., Oxford: Blackwell Publishing, pp701–717.Google Scholar
  33. Van Reeth K, Nauwynck H, Pensaert M. 1996. Dual infections of feeder pigs with porcine reproductive and respiratory syndrome virus followed by porcine respiratory coronavirus or swine in-fluenza virus: a clinical and virological study. Vet Microbiol, 48: 325–335.PubMedCrossRefGoogle Scholar
  34. Webster RG, Rott R. 1987. Influenza virus A pathogenicity: the pivotal role of hemagglutinin. Cell, 50: 665–666.PubMedCrossRefGoogle Scholar
  35. Wei H, Lenz S D, Van Alstine W G, Stevenson G W, Langohr I M, Pogranichniy R M. 2010. Infection of cesarean-derived colostrum-deprived pigs with porcine circovirus type 2 and Swine influenza virus. Comp Med, 60: 45–50.PubMedCentralPubMedGoogle Scholar
  36. Weingartl H M, Berhane Y, Hisanaga T, Neufeld J, Kehler H, Emburry-Hyatt C. 2010. Genetic and pathobiologic characterization of pandemic H1N1 2009 influenza viruses from a naturally infected swine herd. J Virol, 84: 2245–2256.PubMedCentralPubMedCrossRefGoogle Scholar
  37. WHO, 2005. Manual on Animal Influenza Diagnosis and Surveillance, Geneva: World Health Organization.Google Scholar
  38. Yazawa S, Okada M, Ono M, Fujii S, Okuda Y, Shibata I, Kida H. 2004. Experimental dual infection of pigs with an H1N1 swine influenza virus (A/Sw/Hok/2/81) and Mycoplasma hyopneumoniae. Vet Microb, 98: 221–228.CrossRefGoogle Scholar

Copyright information

© Wuhan Institute of Virology, CAS and Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Luisa Fernanda Mancipe Jiménez
    • 1
    Email author
  • Gloria Ramírez Nieto
    • 1
  • Victor Vera Alfonso
    • 1
  • Jairo Jaime Correa
    • 1
    Email author
  1. 1.Laboratory of Animal Virology, Faculty of Veterinary Medicine and Animal ScienceNational University of ColombiaBogotá D.C.Colombia

Personalised recommendations