Skip to main content

Advertisement

Log in

Characterization of a full-length infectious clone of bovine foamy virus 3026

  • Research Article
  • Published:
Virologica Sinica

Abstract

The biological features of most foamy viruses (FVs) are poorly understood, including bovine foamy virus (BFV). BFV strain 3026 (BFV3026) was isolated from the peripheral blood mononuclear cells of an infected cow in Zhangjiakou, China. A full-length genomic clone of BFV3026 was obtained from BFV3026-infected cells, and it exhibited more than 99% amino acid (AA) homology to another BFV strain isolated in the USA. Upon transfection into fetal canine thymus cells, the full-length BFV3026 clone produced viral structural and auxiliary proteins, typical cytopathic effects, and virus particles. These results demonstrate that the full-length BFV3026 clone is fully infectious and can be used in further BFV3026 research.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Achong B G, Mansell P W, Epstein M A, Clifford P. 1971. An unusual virus in cultures from a human nasopharyngeal carcinoma. J Natl Cancer Inst, 46: 299–307.

    PubMed  CAS  Google Scholar 

  • Amborski G F, Lo J L, and Seger C L. 1989. Serological detection of multiple retroviral infections in cattle: Bovine leukemia virus, bovine syncytial virus and bovine visna virus. Vet Microbiol, 20: 247–253.

    Article  PubMed  CAS  Google Scholar 

  • Appleby R C. 1979. Antibodies to bovine syncytial virus in dairy cattle. Vet Rec, 105: 80–81.

    Article  PubMed  CAS  Google Scholar 

  • Bieniasz P D, Rethwilm A, Pitman R, Daniel M D, Chrystie I, McClure M O. 1995. A comparative study of higher primate foamy viruses, including a new virus from a gorilla. Virology, 207: 217–228.

    Article  PubMed  CAS  Google Scholar 

  • Bock M, Heinkelein M, Lindemann D, Rethwilm A. 1998. Cells expressing the human foamy virus (hfv) accessory bet protein are resistant to productive hfv superinfection. Virology, 250: 194–204.

    Article  PubMed  CAS  Google Scholar 

  • Bouillant A M, Ruckerbauer G M, Eaglesome M D, Samagh B S, Singh E L, Hare W C, Randall G C. 1982. Attempts to isolate bovine leukemia and bovine syncytial viruses from blood, uterine flush fluid, unfertilized ova and embryos from infected donor cattle. Ann Rech Vet, 12: 385–395.

    PubMed  CAS  Google Scholar 

  • Broussard S R, Comuzzie A G, Leighton K L, Leland M M, Whitehead E M, Allan J S. 1997. Characterization of new simian foamy viruses from african nonhuman primates. Virology, 237: 349–359.

    Article  PubMed  CAS  Google Scholar 

  • Eastman S W, and Linial M L. 2001. Identification of a conserved residue of foamy virus gag required for intracellular capsid assembly. J Virol, 75: 6857–6864.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  • Enssle J, Jordan I, Mauer B, Rethwilm A. 1996. Foamy virus reverse transcriptase is expressed independently from the gag protein. Proc Natl Acad Sci U S A, 93: 4137–4141.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  • Holzschu D L, Delaney M A, Renshaw R W, Casey J W. 1998. The nucleotide sequence and spliced pol mrna levels of the nonprimate spumavirus bovine foamy virus. J Virol, 72: 2177–2182.

    PubMed Central  PubMed  CAS  Google Scholar 

  • Jacobs R M, Pollari F L, McNab W B, Jefferson B. 1995. A serological survey of bovine syncytial virus in ontario: Associations with bovine leukemia and immunodeficiency-like viruses, production records, and management practices. Can J Vet Res, 59: 271–278.

    PubMed Central  PubMed  CAS  Google Scholar 

  • Jacobs R M, Smith H E, Gregory B, Valli V E, Whetstone C A. 1992. Detection of multiple retroviral infections in cattle and cross-reactivity of bovine immunodeficiency-like virus and human immunodeficiency virus type 1 proteins using bovine and human sera in a western blot assay. Can J Vet Res, 56: 353–359.

    PubMed Central  PubMed  CAS  Google Scholar 

  • Johnson R H, Oginnusi A A, Ladds P W. 1983. Isolations and serology of bovine spumavirus. Aust Vet J, 60: 147.

    Article  PubMed  CAS  Google Scholar 

  • Kehl T, Tan J, Materniak M. 2013. Non-simian foamy viruses: Molecular virology, tropism and prevalence and zoonotic/interspecies transmission. Viruses, 5: 2169–2209.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  • Konvalinka J, Lochelt M, Zentgraf H, Flugel R M, Krausslich H G. 1995. Active foamy virus proteinase is essential for virus infectivity but not for formation of a pol polyprotein. J Virol, 69: 7264–7268.

    PubMed Central  PubMed  CAS  Google Scholar 

  • Lecellier C H, Vermeulen W, Bachelerie F, Giron M L, Saib A. 2002. Intra- and intercellular trafficking of the foamy virus auxiliary bet protein. J Virol, 76: 3388–3394.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  • Linial M. 2000. Why aren’t foamy viruses pathogenic?. Trends Microbiol, 8: 284–289.

    Article  PubMed  CAS  Google Scholar 

  • Linial M L. 1999. Foamy viruses are unconventional retroviruses. J Virol, 73: 1747–1755.

    PubMed Central  PubMed  CAS  Google Scholar 

  • Liu S, Chen H, Chen J, Liang D, Chen Q, Geng Y, and Wood C. 1997. Isolation and identification of a bovine spumavirus isolate 3026. Chin J Virol, 13: 140–145.

    CAS  Google Scholar 

  • Lucas M H, Roberts D H, Parker B N, Wibberley G. 1986. Spread of bovine syncytial virus in a dairy herd over a two year period. Res Vet Sci, 40: 259–263.

    PubMed  CAS  Google Scholar 

  • Mochizuki M, Akuzawa M, and Nagatomo H. 1990. Serological survey of the iriomote cat (felis iriomotensis) in japan. J Wildl Dis, 26: 236–245.

    Article  PubMed  CAS  Google Scholar 

  • Pamba R, Jeronimo C, Archambault D. 1999. Detection of bovine retrospumavirus by the polymerase chain reaction. J Virol Methods, 78: 199–208.

    Article  PubMed  CAS  Google Scholar 

  • Renshaw R W, Casey J W. 1994. Transcriptional mapping of the 3′ end of the bovine syncytial virus genome. J Virol, 68: 1021–1028.

    PubMed Central  PubMed  CAS  Google Scholar 

  • Renshaw R W, Casey J W. 1994. Transcriptional mapping of the 3′ end of the bovine syncytial virus genome. J Virol, 68: 1021–1028.

    PubMed Central  PubMed  CAS  Google Scholar 

  • Tobaly-Tapiero J, Bittoun P, Neves M, Guillemin M C, Lecellier C H, Puvion-Dutilleul F, Gicquel B, Zientara S, Giron M L, de The H, Saib A. 2000. Isolation and characterization of an equine foamy virus. J Virol, 74: 4064–4073.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  • Wang J, Tan J, Guo H, Zhang Q, Jia R, Xu X, Geng Y, Qiao W. 2010. Bovine foamy virus transactivator btas interacts with cellular relb to enhance viral transcription. J Virol, 84: 11888–11897.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  • Wang W, Tan J, Wang J, Chen Q, Geng Y, Qiao W. 2010. Analysis of bovine foamy virus btas mrna transcripts during persistent infection. Virus Genes, 40: 84–93.

    Article  PubMed  CAS  Google Scholar 

  • Yu H, Kong X, Li T, Ma Y, Chen Q, Geng Y. 2003. Characterization of BFV 3026 infection and construction of packaging cell line. Chin J Virol, 19: 330–335.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Wentao Qiao.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bing, T., Yu, H., Li, Y. et al. Characterization of a full-length infectious clone of bovine foamy virus 3026. Virol. Sin. 29, 94–102 (2014). https://doi.org/10.1007/s12250-014-3382-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12250-014-3382-5

Keywords

Navigation