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Calcium phosphate nanoparticles show an effective activation of the innate immune response in vitro and in vivo after functionalization with flagellin

Virologica Sinica volume 29, pages 33–39 (2014)Cite this article

Abstract

For subunit vaccines, adjuvants play a key role in shaping the magnitude, persistence and form of targeted antigen-specific immune response. Flagellin is a potent immune activator by bridging innate inflammatory responses and adaptive immunity and an adjuvant candidate for clinical application. Calcium phosphate nanoparticles are efficient carriers for different biomolecules like DNA, RNA, peptides and proteins. Flagellin-functionalized calcium phosphate nanoparticles were prepared and their immunostimulatory effect on the innate immune system, i.e. the cytokine production, was studied. They induced the production of the proinflammatory cytokines IL-8 (Caco-2 cells) and IL-1β (bone marrow-derived macrophages; BMDM) in vitro and IL-6 in vivo after intraperitoneal injection in mice. The immunostimulation was more pronounced than with free flagellin.

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Authors and Affiliations

  1. Institute of Inorganic Chemistry and Center for Nanointegration Duisburg-Essen (CeNIDE), University of Duisburg-Essen, Essen, 45117, Germany

    Diana Kozlova, Viktoriya Sokolova & Matthias Epple

  2. Mucosal Immunity Research Group, State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China

    Maohua Zhong, Ejuan Zhang, Jingyi Yang, Wei Li, Yi Yang & Huimin Yan

  3. Institute of Medical Microbiology, University Hospital Essen, University of Duisburg-Essen, Essen, 45147, Germany

    Jan Buer & Astrid Maria Westendorf

Authors
  1. Diana Kozlova
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  2. Viktoriya Sokolova
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  3. Maohua Zhong
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  4. Ejuan Zhang
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  5. Jingyi Yang
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  6. Wei Li
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  7. Yi Yang
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  8. Jan Buer
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  9. Astrid Maria Westendorf
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  10. Matthias Epple
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  11. Huimin Yan
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Corresponding authors

Correspondence to Matthias Epple or Huimin Yan.

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Kozlova, D., Sokolova, V., Zhong, M. et al. Calcium phosphate nanoparticles show an effective activation of the innate immune response in vitro and in vivo after functionalization with flagellin. Virol. Sin. 29, 33–39 (2014). https://doi.org/10.1007/s12250-014-3379-0

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  • DOI: https://doi.org/10.1007/s12250-014-3379-0

Keywords

  • calcium phosphate
  • nanoparticle
  • immunostimulation
  • innate immune system
  • flagellin
  • adjuvants